Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses

被引：8

作者：

Pham, Ngan Thi Kim ^{[3
]}

Trinh, Quang Duy ^{[3
]}

Nguyen, Tuan Anh ^{[3
]}

Dey, Shuvra Kanti ^{[3
]}

Phan, Tung Gia ^{[3
]}

Hoang, Le Phuc ^{[4
]}

Khamrin, Pattara ^{[1
,2
]}

Maneekarn, Niwat ^{[5
]}

Okitsu, Shoko ^{[6
,7
]}

Mizuguchi, Masashi ^{[3
]}

Ushijima, Hiroshi ^{[1
,2
]}

机构：

[1] Aino Hlth Sci Ctr, Tokyo, Japan

[2] Aino Univ, Tokyo, Japan

[3] Univ Tokyo, Grad Sch Med, Inst Int Hlth, Dept Dev Med Sci, Tokyo, Japan

[4] Childrens Hosp 1, Dept Gastroenterol, Ho Chi Minh City, Vietnam

[5] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai 50000, Thailand

[6] Aino Sci Ctr, Tokyo, Japan

[7] Aino Coll, Tokyo, Japan

来源：

JOURNAL OF VIROLOGICAL METHODS | 2009年 / 156卷 / 1-2期

关键词：

Aichi virus; Genotype-specific primers; Genotyping; ACUTE GASTROENTERITIS; MOLECULAR CHARACTERIZATION; CHILDREN; BANGLADESH; THAILAND; DIARRHEA; VIETNAM; MEMBER; PICORNAVIRIDAE; DIVERSITY;

D O I：

10.1016/j.jviromet.2008.11.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes A and B of Aichi viruses. Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR products from all 17 samples tested, the newly developed primers could differentiate genotype A from genotype B, and all matched those obtained by nucleotide sequencing of the capsid regions. The nested PCR method using genotype-specific primers is useful and can be used for genotyping of Aichi viruses isolated from epidemiological studies. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：107 / 110

页数：4

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