Permeabilization of Ochrobactrum anthropi SY509 cells with organic solvents for whole cell biocatalyst

被引：25

作者：

Choi, KO

Song, SH

Yoo, YJ ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem Engn, Seoul 151742, South Korea

[2] Seoul Natl Univ, Interdisciplinary Program Biochem Engn & Biotechn, Seoul 151742, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2004年 / 9卷 / 03期

关键词：

permeabilization; permeability barrier; organic solvent; Ochrobactrum anthropi; phospholipids;

D O I：

10.1007/BF02942284

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Permeabilization is known to overcome cell membrane barriers of whole cell biocatalysts. The use of organic solvents is advantageous in terms of cost, simplicity, and efficiency. In this study, Ochrobactrum anthropi SY509 was permeabilized with various organic solvents. Treatment with organic solvents resulted in lower permeability barriers due to falling out lipids of the cell membrane. Therefore, permeabilized cells showed higher enzyme activity with no cell viability. Among various organic solvents, 0.5% (v/v) chloroform was selected as the most efficient permeabilizing reagent. Changes in the cell membrane structure were observed and the residual amounts of phospholipids of the cell membrane were measured to investigate the mechanism of the improved permeability.

引用

页码：147 / 150

页数：4

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