Complete nucleotide sequence of a novel partitivirus from Brassica campestris L. ssp. chinensis

被引:4
|
作者
Tang, Liguang [1 ,2 ]
Song, Liping [1 ]
Lin, Chufa [1 ]
Wang, Bincai [1 ]
Lin, Jiazao [1 ]
Gao, Changbin [1 ]
Wang, Aihua [1 ]
机构
[1] Wuhan Acad Agr Sci, Wuhan Vegetable Res Inst, Wuhan, Hubei, Peoples R China
[2] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China
关键词
MOLECULAR CHARACTERIZATION; CLONING; GENOME; VIRUS;
D O I
10.1007/s00705-021-05041-x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In the present work, we report the discovery and complete genome sequence of a novel partitivirus identified from Brassica campestris L. ssp. chinensis, which we have named "Brassica campestris chinensis cryptic virus 1" (BCCV1). Next-generation sequencing (NGS) combined with adapter-ligation-mediated amplification allowed assembly of the full-length genome sequence of BCCV1. The genome of BCCV1 contains two dsRNA segments, dsRNA1 (1595 bp) and dsRNA2 (1591 bp), which encode a conserved RNA-dependent RNA polymerase (RdRp) and a putative capsid protein (CP), respectively. Homology searches and phylogenetic analysis of the 479-aa RdRp and 438-aa CP showed that BCCV1 is a new member of the genus Deltapartitivirus, family Partitiviridae. This is the first report of the identification of a member of the family Partitiviridae in Brassica campestris L. ssp. chinensis.
引用
收藏
页码:1775 / 1778
页数:4
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