Complete nucleotide sequence of a novel partitivirus from Brassica campestris L. ssp. chinensis

被引：4

作者：

Tang, Liguang ^{[1
,2
]}

Song, Liping ^{[1
]}

Lin, Chufa ^{[1
]}

Wang, Bincai ^{[1
]}

Lin, Jiazao ^{[1
]}

Gao, Changbin ^{[1
]}

Wang, Aihua ^{[1
]}

机构：

[1] Wuhan Acad Agr Sci, Wuhan Vegetable Res Inst, Wuhan, Hubei, Peoples R China

[2] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2021年 / 166卷 / 06期

关键词：

MOLECULAR CHARACTERIZATION; CLONING; GENOME; VIRUS;

D O I：

10.1007/s00705-021-05041-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In the present work, we report the discovery and complete genome sequence of a novel partitivirus identified from Brassica campestris L. ssp. chinensis, which we have named "Brassica campestris chinensis cryptic virus 1" (BCCV1). Next-generation sequencing (NGS) combined with adapter-ligation-mediated amplification allowed assembly of the full-length genome sequence of BCCV1. The genome of BCCV1 contains two dsRNA segments, dsRNA1 (1595 bp) and dsRNA2 (1591 bp), which encode a conserved RNA-dependent RNA polymerase (RdRp) and a putative capsid protein (CP), respectively. Homology searches and phylogenetic analysis of the 479-aa RdRp and 438-aa CP showed that BCCV1 is a new member of the genus Deltapartitivirus, family Partitiviridae. This is the first report of the identification of a member of the family Partitiviridae in Brassica campestris L. ssp. chinensis.

引用

页码：1775 / 1778

页数：4

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