Chitosan oligosaccharide inhibits EGF-induced cell growth possibly through blockade of epidermal growth factor receptor/imitogen-activated protein kinase pathway

被引：17
|
作者：
Xu, Qingsong [1 ]
Wang, Wenjing [2 ]
Yang, Wen [1 ]
Du, Yuguang [3 ]
Song, Linsheng [1 ]
机构：
[1] Dalian Ocean Univ, Coll Fisheries & Life Sci, 52 Heishijiao St, Dalian 116023, Peoples R China
[2] Dalian Elite Analyt Instruments Co Ltd, Dalian 116023, Peoples R China
[3] Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2017年 / 98卷
基金：
中国国家自然科学基金;
关键词：
Chitosan oligosaccharide; Epidermal growth factor; Cell growth; TO-MESENCHYMAL TRANSITION; TUMOR MICROENVIRONMENT; CHITOOLIGOSACCHARIDES; CHITIN; ROLES;
D O I：
10.1016/j.ijbiomac.2017.02.021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chitosan oligosaccharide (COS) has been shown to regulate various cellular and biological functions. Epidermal growth factor (EGF) plays a significant role in tumorigenesis and invasiveness of some cancers. The aim of this study was to evaluate the effects of COS on EGF-induced cell growth and the further mechanisms. The results demonstrated that COS could inhibit EGF-induced epithelial GE11 cells proliferation in a dose dependent manner. In addition, EGF stimulated the epithelial cells to undergo morphological alteration, exhibiting mesenchymal cells higher metastatic and invasive potential, however, COS could partly suppress aforementioned morphological change. Signal transduction studies indicated COS repressed epidermal growth factor receptor (EGFR) phosphorylation and mitogen-activated protein kinase (MAPK) activation, but not Grb2, Ras, and Raf. Taken together, chitosan oligosaccharide inhibited EGF-induced cell growth and migration through blockade of the EGFR/MAPK signal transduction pathway. (C) 2017 Elsevier B.V. All rights reserved.
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页码：502 / 505
页数：4
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