Development and characterization of microsatellite markers for the Brazil nut tree Bertholletia excelsa Humb. & Bonpl. (Lecythidaceae)

被引：13
|
作者：
Reis, Alessandra M. M. [1 ]
Braga, Aline C. [1 ]
Lemes, Maristerra R. [2 ]
Gribel, Rogerio [2 ]
Collevatti, Rosane G. [1 ]
机构：
[1] Univ Catolica Brasilia, Programa Posgrad Ciencias Genom & Biotecnol, BR-70790160 Brasilia, DF, Brazil
[2] INPA, Lab Genet & Biol Reprod Plantas, BR-69083000 Manaus, Amazonas, Brazil
来源：
MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 03期
关键词：
Amazonia; Bertholletia excelsa; Brazil nut; Lecythidaceae; microsatellite; tropical tree; MATING SYSTEM; GENETIC-STRUCTURE; POPULATION;
D O I：
10.1111/j.1755-0998.2008.02481.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Twelve polymorphic microsatellite markers were developed for the Brazil nut (Bertholletia excelsa), one of the most valuable non-timber forest products from the Amazon, based on enrichment protocol. Six to 18 (mean 10.4) alleles per locus were identified and the expected heterozygosity ranged from 0.663 to 0.923 based on a screen of 40 individuals from one population of B. excelsa. The combined probabilities of genetic identity (8.39 x 10(-17)) and paternity exclusion (0.999999) indicated that multilocus genotypes are likely to be unique allowing precise analyses of genetic structure, gene flow, and mating system of this economically important species.
引用
收藏
页码：920 / 923
页数：4
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