Importance of the epa Locus of Enterococcus faecalis OG1RF in a Mouse Model of Ascending Urinary Tract Infection

被引：28
|
作者：
Singh, Kavindra V. [1 ,2 ]
Lewis, Roshan J. [1 ,2 ]
Murray, Barbara E. [1 ,2 ,3 ]
机构：
[1] Univ Texas Med Sch Houston, Div Infect Dis, Ctr Study Emerging & Reemerging Pathogens, Houston, TX 77030 USA
[2] Univ Texas Med Sch, Dept Internal Med, Houston, TX 77030 USA
[3] Univ Texas Med Sch, Dept Mol Genet & Microbiol, Houston, TX 77030 USA
来源：
JOURNAL OF INFECTIOUS DISEASES | 2009年 / 200卷 / 03期
关键词：
ENDOCARDITIS; SERUM; PATHOGENESIS; RESISTANCE; EXPRESSION; CLUSTER; GENES;
D O I：
10.1086/600124
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Previously, TX5179, a disruption mutant of the enterococcal polysaccharide antigen (epa) gene cluster of Enterococcus faecalis strain OG1RF was shown to be attenuated in translocation, biofilm, mouse peritonitis and was more susceptible to polymorphonuclear leukocyte phagocytic killing. Here, wild-type E. faecalis OG1RF and TX5179 strains were tested in a mixed-infection (inoculum, similar to 1:1) mouse urinary tract infection model. Wild-type OG1RF outnumbered TX5179 in the kidneys (P <.001) and bladder (P <.001). In conclusion, the epa locus of E. faecalis OG1RF contributes to murine urinary tract infection and is the first such enterococcal polysaccharide locus shown to be important in this site.
引用
收藏
页码：417 / 420
页数：4
相关论文
共 50 条
  • [11] Large scale variation in Enterococcus faecalis illustrated by the genome analysis of strain OG1RF
    Agathe Bourgogne
    Danielle A Garsin
    Xiang Qin
    Kavindra V Singh
    Jouko Sillanpaa
    Shailaja Yerrapragada
    Yan Ding
    Shannon Dugan-Rocha
    Christian Buhay
    Hua Shen
    Guan Chen
    Gabrielle Williams
    Donna Muzny
    Arash Maadani
    Kristina A Fox
    Jason Gioia
    Lei Chen
    Yue Shang
    Cesar A Arias
    Sreedhar R Nallapareddy
    Meng Zhao
    Vittal P Prakash
    Shahreen Chowdhury
    Huaiyang Jiang
    Richard A Gibbs
    Barbara E Murray
    Sarah K Highlander
    George M Weinstock
    Genome Biology, 9
  • [12] Loss-of-Function Mutations in epaR Confer Resistance to φNPV1 Infection in Enterococcus faecalis OG1RF
    Ho, Khang
    Huo, Wenwen
    Pas, Savannah
    Dao, Ryan
    Palmer, Kelli L.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2018, 62 (10)
  • [13] ccfA, the genetic determinant for the cCF10 peptide pheromone in Enterococcus faecalis OG1RF
    Antiporta, MH
    Dunny, GM
    JOURNAL OF BACTERIOLOGY, 2002, 184 (04) : 1155 - 1162
  • [14] Characterization of fsr, a regulator controlling expression of gelatinase and serine protease in Enterococcus faecalis OG1RF
    Qin, X
    Singh, KV
    Weinstock, GM
    Murray, BE
    JOURNAL OF BACTERIOLOGY, 2001, 183 (11) : 3372 - 3382
  • [15] Relative Contributions of Enterococcus faecalis OG1RF Sortase-Encoding Genes, srtA and bps (srtC), to Biofilm Formation and a Murine Model of Urinary Tract Infection (vol 75, pg 5399, 2007)
    Kemp, Kelvin D.
    Singh, Kavindra V.
    Nallapareddy, Sreedhar R.
    Murray, Barbara E.
    INFECTION AND IMMUNITY, 2010, 78 (01) : 562 - 562
  • [16] Sortase-encoding genes, srtA and srtC, mediate Enterococcus faecalis OG1RF persistence in the Helicoverpa zea gastrointestinal tract
    Jackson, Jerreme J.
    Heyer, Samantha
    Bell, Geneva
    FRONTIERS IN MICROBIOLOGY, 2024, 15
  • [17] Enterococcal aggregation substance and binding substance are not major contributors to urinary tract colonization by Enterococcus faecalis in a mouse model of ascending unobstructed urinary tract infection
    Johnson, JR
    Clabots, C
    Hirt, H
    Waters, C
    Dunny, G
    INFECTION AND IMMUNITY, 2004, 72 (04) : 2445 - 2448
  • [18] Comparative Biofilm Assays Using Enterococcus faecalis OG1RF Identify New Determinants of Biofilm Formation
    Willett, L. E. Julia
    Dale, L. Jennifer
    Kwiatkowski, M. Lucy
    Powers, L. Jennifer
    Korir, L. Michelle
    Kohli, Rhea
    Barnes, M. T. Aaron
    Dunny, M. Gary
    MBIO, 2021, 12 (03):
  • [19] The Two-Component System GrvRS (EtaRS) Regulates ace Expression in Enterococcus faecalis OG1RF
    Roh, Jung Hyeob
    Singh, Kavindra V.
    La Rosa, Sabina Leanti
    Cohen, Ana Luisa V.
    Murray, Barbara E.
    INFECTION AND IMMUNITY, 2015, 83 (01) : 389 - 395
  • [20] Phosphotransferase systems in Enterococcus faecalis OG1RF enhance anti-stress capacity in vitro and in vivo
    Peng, Zhen
    Ehrmann, Matthias A.
    Waldhuber, Anna
    Niemeyer, Christine
    Miethke, Thomas
    Frick, Julia-Stefanie
    Xiong, Tao
    Vogel, Rudi F.
    RESEARCH IN MICROBIOLOGY, 2017, 168 (06) : 558 - 566
12345