NF-κB inhibits transcription of the H+-K+-ATPase α2-subunit gene:: role of histone deacetylases

被引:61
|
作者
Zhang, WZ
Kone, BC
机构
[1] Univ Texas, Sch Med, Dept Internal Med, Houston, TX 77030 USA
[2] Univ Texas, Sch Med, Dept Integrat Biol Pharmacol & Physiol, Houston, TX 77030 USA
关键词
kidney; colon; trichostatin A; promoter;
D O I
10.1152/ajprenal.00156.2002
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The H+-K+-ATPase alpha(2) (HKalpha(2)) gene plays a central role in potassium homeostasis, yet little is known about its transcriptional control. We recently demonstrated that the proximal promoter confers basal transcriptional activity in mouse inner medullary collecting duct 3 cells. We sought to determine whether the kappaB DNA binding element at -104 to -94 influences basal HKalpha(2) gene transcription in these cells. Recombinant NF-kappaB p50 footprinted the region -116/-94 in vitro. Gel shift and supershift analysis revealed NF-kappaB p50- and p65-containing DNA-protein complexes in nuclear extracts of mouse inner medullary collecting duct 3 cells. A promoter-luciferase construct with a mutated -104/-94 NF-kappaB element exhibited higher activity than the wild-type promoter in transfection assays. Overexpression of NF-kappaB p50, p65, or their combination transrepressed the HKalpha(2) promoter. The histone deacetylase (HDAC) inhibitor trichostatin A partially reversed NF-kappaB-mediated trans-repression of the HKalpha(2) promoter. HDAC6 overexpression inhibited HKalpha(2) promoter activity, and HDAC6 coimmunoprecipitated with NF-kappaB p50 and p65. These results suggest that HDAC6, recruited to the DNA protein complex, acts with NF-kappaB to suppress HKalpha(2) transcription and identify NF-kappaB p50 and p65 as novel binding partners for HDAC6.
引用
收藏
页码:F904 / F911
页数:8
相关论文
共 50 条
  • [21] The Na,K-ATPase ß2-subunit modulates epidermal growth factor signaling through NF2/Merlin.
    Litan, A.
    Li, Z.
    Vagin, O.
    Langhans, S. A.
    MOLECULAR BIOLOGY OF THE CELL, 2017, 28
  • [22] Helicobacter pylori-induced H, K-ATPase α-subunit gene repression is mediated by NF-κB p50 homodimer promoter binding
    Saha, Arindam
    Hammond, Charles E.
    Trojanowska, Maria
    Smolka, Adam J.
    AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2008, 294 (03): : G795 - G807
  • [23] Ginsenoside compound K inhibits NF- κ B by targeting Annexin A2
    Wang, Yushi
    Jin, Yinghua
    CANCER SCIENCE, 2018, 109 : 230 - 230
  • [24] NF-κB-dependent repression of Sox18 transcription factor requires the epigenetic regulators histone deacetylases 1 and 2 in acute lung injury
    Zemskov, Evgeny A. A.
    Gross, Christine M. M.
    Aggarwal, Saurabh
    Zemskova, Marina A. A.
    Wu, Xiaomin
    Gu, Chenxin
    Wang, Ting
    Tang, Haiyang
    Black, Stephen M. M.
    FRONTIERS IN PHYSIOLOGY, 2022, 13
  • [25] EVOLUTION OF THE NA,K-ATPASE AND H,K-ATPASE BETA-SUBUNIT GENE FAMILY - STRUCTURE OF THE MURINE NA,K-ATPASE BETA-2 SUBUNIT GENE
    SHYJAN, AW
    CANFIELD, VA
    LEVENSON, R
    GENOMICS, 1991, 11 (02) : 435 - 442
  • [26] Glycyrrhizin inhibits NF-κ B-dependent gene transcription and lung inflammation not through glucocorticoid receptor
    Takei, Hironori
    Nishimoto, Hiroki
    Hisatsune, Akinori
    Miyata, Takeshi
    Isohama, Yoichiro
    JOURNAL OF PHARMACOLOGICAL SCIENCES, 2007, 103 : 114P - 114P
  • [27] Nitric oxide inhibits transcription of the Na+-K+-ATPase α1-subunit gene in an MTAL cell line
    Kone, BC
    Higham, S
    AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1999, 276 (04) : F614 - F621
  • [28] Metformin inhibits histone H2B monoubiquitination and downstream gene transcription in human breast cancer cells
    Du, Yu
    Zheng, Haiyan
    Wang, Jiang
    Ren, Ye
    Li, Mi
    Gong, Chen
    Xu, Fei
    Yang, Caihong
    ONCOLOGY LETTERS, 2014, 8 (02) : 809 - 812
  • [29] Modulation of NF-κB-dependent gene expression by H2O2 depends on the NF-κB affinity to κB sites
    De Oliveira-Marques, Virginia
    Antunes, Fernando
    Marinho, H. Susana
    Cyrne, Luisa
    FREE RADICAL BIOLOGY AND MEDICINE, 2007, 43 : S149 - S150
  • [30] STRUCTURAL ORGANIZATION AND TRANSCRIPTION OF THE MOUSE GASTRIC H+,K+-ATPASE BETA-SUBUNIT GENE
    CANFIELD, VA
    LEVENSON, R
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (18) : 8247 - 8251