Region Required for Protein Expression from the Stop-Start Pentanucleotide in the M Gene of Influenza B Virus

被引：11

作者：

Hatta, Masato ^{[1
]}

Kohlmeier, Candice K. ^{[1
]}

Hatta, Yasuko ^{[1
]}

Ozawa, Makoto ^{[1
]}

Kawaoka, Yoshihiro ^{[1
,2
,3
,4
]}

机构：

[1] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA

[2] Univ Tokyo, Dept Microbiol & Immunol, Div Virol, Tokyo 1088639, Japan

[3] Univ Tokyo, Int Res Ctr Infect Dis, Inst Med Sci, Tokyo 1088639, Japan

[4] Japan Sci & Technol Agcy, ERATO Infect Induced Host Responses Project, Saitama 3320012, Japan

来源：

JOURNAL OF VIROLOGY | 2009年 / 83卷 / 11期

关键词：

COUPLED TRANSLATION; REINITIATION; CALICIVIRUS; GENERATION; RNA;

D O I：

10.1128/JVI.00180-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Segment 7 of influenza B virus encodes two proteins, M1 and BM2. BM2 is expressed from a stop-start pentanucleotide, in which the BM2 initiation codon overlaps with the M1 stop codon. Here, we demonstrate that 45 nucleotides of the 3' end of the M1 coding region, but not the 5' end of the BM2 coding region, are sufficient for the efficient expression of the downstream protein. Placing these 45 nucleotides and the stop-start pentanucleotide in between the coding sequences induced the expression of at least three noninfluenza proteins, suggesting the utility of this system for expressing multiple proteins from one mRNA.

引用

页码：5939 / 5942

页数：4

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