Cloning and sequencing of the maltohexaose-producing amylase gene of Klebsiella pneumoniae

被引：14
|
作者：
Momma, M [1 ]
机构：
[1] Natl Inst Agrobiol Resources, Dept Biotechnol, Tsukuba, Ibaraki 3058602, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 02期
关键词：
gene cloning; amylase; maltohexaose; Klebsiella pneumoniae;
D O I：
10.1271/bbb.64.428
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The molecular characterization of the maltohexaose-producing amylase gene of Klebsiella pneumoniae revealed an open reading frame in which 2,031 base pairs encode a protein of 677 amino acids with a calculated molecular weight of 75,921, The amylase gene had high similarities of 73.6% in DNA sequence and 79.3% in deduced amino acid sequence with the periplasmic alpha-amylase MalS gene of Escherichia coli.
引用
收藏
页码：428 / 431
页数：4
相关论文
共 50 条
  • [21] CLONING, SEQUENCING AND HETEROLOGOUS EXPRESSION OF A KLEBSIELLA-PNEUMONIAE GENE ENCODING AN FAD-INDEPENDENT ACETOLACTATE SYNTHASE
    PENG, HL
    WANG, PY
    WU, CM
    HWANG, DC
    CHANG, HY
    GENE, 1992, 117 (01) : 125 - 130
  • [22] Improvement of the quality and shelf life of wheat bread by a maltohexaose producing α-amylase
    Zhang, Lei
    Li, Zhoukun
    Qiao, Yan
    Zhang, Yajuan
    Zheng, Wenwen
    Zhao, Yuqiang
    Huang, Yan
    Cui, Zhongli
    JOURNAL OF CEREAL SCIENCE, 2019, 87 : 165 - 171
  • [23] CLONING AND SEQUENCE-ANALYSIS OF GYRA GENE OF KLEBSIELLA-PNEUMONIAE
    DIMRI, GP
    DAS, HK
    NUCLEIC ACIDS RESEARCH, 1990, 18 (01) : 151 - 156
  • [24] Molecular cloning and kinetic characterization of an aldehyde dehydrogenase gene in Klebsiella pneumoniae
    Ma, LC
    Lee, SL
    Lee, CZ
    Yin, SJ
    Wang, JT
    JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION, 2005, 104 (04) : 221 - 230
  • [25] Epidemiology of Klebsiella pneumoniae carbapenemase (KPC) producing Klebsiella pneumoniae in a German hospital
    Wendt, C.
    Schuett, S.
    Dalpke, A.
    Zimmermann, S.
    Trierweiler-Hauke, B.
    Konrad, M.
    Mieth, M.
    Weigand, M.
    Hoppe-Tichy, T.
    Jonas, D.
    INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 298 : 34 - 35
  • [26] Infection with Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae in cancer patients
    Freire, M. P.
    Pierrotti, L. C.
    Filho, H. H. C.
    Ibrahim, K. Y.
    Magri, A. S. G. K.
    Bonazzi, P. R.
    Hajar, L.
    Diz, M. P. E.
    Pereira, J.
    Hoff, P. M.
    Abdala, E.
    EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2015, 34 (02) : 277 - 286
  • [27] Infection with Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae in cancer patients
    M. P. Freire
    L. C. Pierrotti
    H. H. C. Filho
    K. Y. Ibrahim
    A. S. G. K. Magri
    P. R. Bonazzi
    L. Hajar
    M. P. E. Diz
    J. Pereira
    P. M. Hoff
    E. Abdala
    European Journal of Clinical Microbiology & Infectious Diseases, 2015, 34 : 277 - 286
  • [28] Detection Sequencing NDM and blaOXA Genes in Metallo-β-Lactamase Producing Klebsiella Pneumoniae
    Hussain, Rahma Ali Alhadi
    Kzar, Ashwak Jasim
    Hamza, Alaa Salim
    JOURNAL OF PIONEERING MEDICAL SCIENCES, 2023, 12 (03): : 33 - 37
  • [29] ISOLATION AND ACTION PATTERN OF MALTOHEXAOSE PRODUCING AMYLASE FROM AEROBACTER-AEROGENES
    KAINUMA, K
    ITO, T
    SUZUKI, S
    KOBAYASH.S
    FEBS LETTERS, 1972, 26 (01) : 281 - &
  • [30] CLONING AND PARTIAL SEQUENCING OF AN AMYLASE GENE AND ITS INTRODUCTION INTO STREPTOMYCES BY TRANSPOSITION
    OLSEN, MK
    PATT, TE
    TOMICH, CSC
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1988, 196 : 89 - MBTD
12345