Cloning and sequencing of the maltohexaose-producing amylase gene of Klebsiella pneumoniae

被引：14
|
作者：
Momma, M [1 ]
机构：
[1] Natl Inst Agrobiol Resources, Dept Biotechnol, Tsukuba, Ibaraki 3058602, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 02期
关键词：
gene cloning; amylase; maltohexaose; Klebsiella pneumoniae;
D O I：
10.1271/bbb.64.428
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The molecular characterization of the maltohexaose-producing amylase gene of Klebsiella pneumoniae revealed an open reading frame in which 2,031 base pairs encode a protein of 677 amino acids with a calculated molecular weight of 75,921, The amylase gene had high similarities of 73.6% in DNA sequence and 79.3% in deduced amino acid sequence with the periplasmic alpha-amylase MalS gene of Escherichia coli.
引用
收藏
页码：428 / 431
页数：4
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