Stability and catalytic kinetics of acid phosphatase immobilized on composite beads of chitosan and activated clay

被引：46
|
作者：
Chang, MY [1 ]
Juang, RS
机构：
[1] Natl United Univ, Dept Chem Engn, Miaoli 360, Taiwan
[2] Yuan Ze Univ, Dept Chem Engn, Chungli 320, Taiwan
来源：
PROCESS BIOCHEMISTRY | 2004年 / 39卷 / 09期
关键词：
acid phosphatase; immobilization; composite beads; chitosan; activated clay; catalytic kinetics; stability;
D O I：
10.1016/S0032-9592(03)00221-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The stability of acid phosphatase immobilized on composite beads was studied. The beads were prepared from equal weights of cuttlebone chitosan and activated clay and were cross-linked with glutaraldehyde. The immobilized enzyme maintained 90% of its original activity after 50 times of reuse. The immobilized acid phosphatase revealed acceptable thermal and pH stabilities over a broad experimental range. Thermal deactivation of immobilized enzyme was also examined by first-order kinetics and the deactivation energy was determined. The kinetics of a model reaction catalyzed by the immobilized acid phosphatase was finally investigated by the Michaelis-Menten equation. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1087 / 1091
页数：5
相关论文
共 50 条
  • [21] Preparation of immobilized neutral protease on chitosaneous beads and their properties, stability and application to hydrolysis of chitosan
    Du Yumin
    Jin, Li
    Tao, Feng
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2005, 230 : U4043 - U4044
  • [22] CHITOSAN BEADS IMMOBILIZED SCHIZOPHYLLUM COMMUNE IBL-06 LIGNIN PEROXIDASE WITH NOVEL THERMO STABILITY, CATALYTIC AND DYE REMOVAL PROPERTIES
    Sofia, P.
    Asgher, M.
    Shahid, M.
    Randhawa, M. A.
    [J]. JOURNAL OF ANIMAL AND PLANT SCIENCES, 2016, 26 (05): : 1451 - 1463
  • [23] Adsorption of As and Pb by Stone Powder/Chitosan/Maghemite Composite Beads (SCM Beads): Kinetics and Column Study
    Song, Gunho
    Oh, Sanghwa
    [J]. PROCESSES, 2023, 11 (02)
  • [24] Stability of β-galactosidase immobilized on composite microspheres of artemisia seed gum and chitosan
    Zhang, Ji
    Zhang, Shengtang
    Wang, Yunpu
    Gao, Sufang
    [J]. POLYMER COMPOSITES, 2008, 29 (01) : 9 - 14
  • [25] Immobilization of acid phosphatase from Vigna aconitifolia seeds on chitosan beads and its characterization
    Srivastava, Pramod Kumar
    Anand, Asha
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2014, 64 : 150 - 154
  • [26] Purification of trypsin inhibitor from sweet potato by applying immobilized trypsin on glutaraldehyde activated chitosan beads
    Kuen-Shan Jaw
    Kow-Jen Duan
    Ming-Tse Lin
    [J]. JOURNAL OF THE CHINESE INSTITUTE OF CHEMICAL ENGINEERS, 2006, 37 (02): : 125 - 129
  • [27] Kinetics and thermodynamics of Rhodamine B adsorption by gelatin/activated carbon composite beads
    Hayeeye, Fareeda
    Sattar, Memoon
    Chinpa, Watchanida
    Sirichote, Orawan
    [J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2017, 513 : 259 - 266
  • [28] COMPARISON BETWEEN THE ENZYMIC AND THE IMMUNOLOGICAL DEMONSTRATION OF PROSTATIC ACID-PHOSPHATASE IMMOBILIZED ON AGAROSE BEADS
    RICHARDSON, TC
    HEYDERMAN, E
    [J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1984, 12 (05) : 832 - 833
  • [29] Decolorization and detoxification of textile effluent by immobilized laccase-ACS into chitosan-clay composite beads using a packed bed reactor system: An ecofriendly approach
    Mehandia, Seema
    Ahmad, Shabeer
    Sharma, S. C.
    Arya, Shailendra Kumar
    [J]. JOURNAL OF WATER PROCESS ENGINEERING, 2022, 47
  • [30] Catalytic behaviour of acid phosphatase immobilized on natural supports in the presence of manganese or molybdenum
    Rosas, Anali
    Mora, Maria de la Luz
    Jara, Alejandra A.
    Lopez, Roxana
    Rao, Maria A.
    Gianfreda, Liliana
    [J]. GEODERMA, 2008, 145 (1-2) : 77 - 83
12345