SOD3 Acts as a Tumor Suppressor in PC-3 Prostate Cancer Cells via Hydrogen Peroxide Accumulation

被引:3
|
作者
Kim, Jungim [1 ]
Mizokami, Atsushi [1 ]
Shin, Minkyoung [1 ]
Izumi, Kouji [1 ]
Konaka, Hiroyuki [1 ]
Kadono, Yoshifumi [1 ]
Kitagawa, Yasuhide [1 ]
Keller, Evan T. [2 ]
Zhang, Jian [3 ]
Namiki, Mikio [1 ]
机构
[1] Kanazawa Univ, Grad Sch Med Sci, Dept Integrat Canc Therapy & Urol, Kanazawa, Ishikawa 9208640, Japan
[2] Univ Michigan, Dept Urol & Pathol, Ann Arbor, MI 48109 USA
[3] Guangxi Med Univ, Ctr Translat Med, Nanning, Guangxi, Peoples R China
关键词
SOD3; prostate cancer; proliferation; migration; hydrogen peroxide; DNA damage; EXTRACELLULAR-SUPEROXIDE-DISMUTASE; MATRIX METALLOPROTEINASE-2; OXIDATIVE STRESS; ANTIOXIDANT ENZYMES; CARCINOMA-CELLS; DOWN-REGULATION; REDOX STATE; EXPRESSION; INHIBITION; GROWTH;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: The functions of superoxide dismutase-3 (SOD3), which acts on the cell surface and protects cells from oxidative stress, remain uncertain in the progression of prostate cancer. Materials and Methods: To verify SOD3 expression in human prostate tissue, immunohistochemistry was performed using tissue microarrays. To investigate the effects of SOD3 on proliferation, migration, and invasion, SOD3 was overexpressed and recombinant SOD3 was employed in PC-3 prostate cancer cells. H2O2 levels, reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, catalase activity, and 8-oxo-2'-deoxyguanosine (8-OHdG) were estimated in SOD3-overexpressing PC-3 cells. Results: Immunohistochemistry revealed reduced expression of SOD3 in prostate cancer tissue. SOD3 overexpression in PC-3 cells inhibited cell proliferation, migration, and invasion. Recombinant SOD3 had the same effect. H2O2 accumulation was increased by SOD3 overexpression, GSH/GSSG ratio was decreased, and catalase activity was decreased. DNA damage in SOD3-overexpressing cells was confirmed by 8-OHdG elevation. Conclusion: Since SOD3 acts as a tumor suppressor, SOD3 overexpression and recombinant SOD3 might lead to treatment for prostate cancer.
引用
收藏
页码:2821 / 2831
页数:11
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