High-field electron spin resonance of spin labels in membranes

被引：32
|
作者：
Marsh, D [1 ]
Kurad, D
Livshits, VA
机构：
[1] Max Planck Inst Biophys Chem, Spekt Abt, D-37070 Gottingen, Germany
[2] Russian Acad Sci, Ctr Photochem, Moscow 117427, Russia
来源：
CHEMISTRY AND PHYSICS OF LIPIDS | 2002年 / 116卷 / 1-2期
关键词：
spin label; electron spin resonance; lipid mobility; rotational diffusion; lateral ordering; membrane polarity;
D O I：
10.1016/S0009-3084(02)00022-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
High-field electron spin resonance (ESR) spectroscopy is currently undergoing rapid development. This considerably increases the versatility of spin labelling which, at conventional field strengths, is already well established as a powerful physical technique in membrane biology. Among the unique advantages offered by high-field spectroscopy. particularly for spin-labelled lipids, are sensitivity to non-axial rotation and lateral ordering, a better orientational selection, an extended application to rotational dynamics, and an enhanced sensitivity to environmental polarity. These areas are treated in some depth, along with a detailed consideration of recent developments in the investigation of transmembrane polarity profiles. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：93 / 114
页数：22
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