E-cadherin and periostin in early detection and progression of diabetic nephropathy: epithelial-to-mesenchymal transition

被引:34
|
作者
El-Dawla, Nada M. Qamar [1 ]
Sallam, Al-Aliaa M. [2 ]
El-Hefnawy, Mohamed H. [3 ]
El-Mesallamy, Hala O. [2 ]
机构
[1] Modern Univ Technol & Informat, Fac Pharm, Biochem Dept, Cairo, Egypt
[2] Ain Shams Univ, Fac Pharm, Biochem Dept, Cairo 11566, Egypt
[3] Natl Inst Diabet & Endocrinol NIDE, Cairo, Egypt
关键词
Diabetic nephropathy; E-cadherin; EMT; Periostin; RENAL INJURY; BIOMARKER; IDENTIFICATION; MECHANISMS; EXPRESSION; TISSUE;
D O I
10.1007/s10157-019-01744-3
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Background Diabetic nephropathy (DN) is a severe complication of diabetes mellitus (DM). Many mechanisms are involved in its development; one of these mechanisms is epithelial-to-mesenchymal transition (EMT). During EMT, losing of the epithelial biomarkers like E-cadherin and increasing of mesenchymal biomarkers like periostin are very characteristic. Methods The study included 19 healthy controls and 71 DN patients categorized according to their urinary albumin-to-creatinine ratio (UACR) into 19 normoalbuminuric (UACR < 30 mg/g), 37 microalbuminuric (UACR 30-300 mg/g), and 15 macroalbuminuric (UACR > 300 mg/g) patients. Fasting plasma glucose (FPG), glycated hemoglobin (HbA(1C)%), serum creatinine (Cr), and urea were measured. E-cadherin and periostin were measured by ELISA and compared among groups. Results Concerning E-cadherin levels, in comparison to control group, there were significantly decreased in all groups (0.94, 0.52, and 0.14 ng/mL in normoalbuminuria, microalbuminuria, and macroalbuminuria groups; respectively). For periostin levels, nonsignificant increase in normoalbuminuria (0.32 ng/mL) than control group (0.3 ng/mL) was observed. There was a significant increase in other groups with the highest values in macroalbuminuria group (1.66 ng/mL). E-cadherin and periostin were correlated with each other (r = -0.353, P < 0.001). UACR was negatively correlated with E-cadherin and positively correlated with periostin. ROC curve analyses showed that the AUC to diagnose established microalbuminuria using E-cadherin was 0.998 (95% CI 0.932-1), and using periostin was 0.833 (95% CI 0.709-0.919). Conclusion Serum E-cadherin and periostin could be considered as reliable biomarkers involved in DN pathogenesis and linked to its stages.
引用
收藏
页码:1050 / 1057
页数:8
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