Alterations in GHRH binding and GHRH receptor mRNA in the pituitary of adult dw/dw rats

被引：3
|
作者：
Gardi, J
Speth, RC [1 ]
Taishi, P
Kacsóh, B
Obal, F
Krueger, JM
机构：
[1] Washington State Univ, Dept Vet & Comparat Anat Pharmacol & Physiol, Pullman, WA 99164 USA
[2] Mercer Univ, Sch Med, Div Basic Med Sci, Macon, GA 31207 USA
[3] Mercer Univ, Sch Med, Dept Pediat, Macon, GA 31207 USA
[4] Univ Szeged, Albert Szent Gyorgyi Med Ctr, Dept Physiol, H-6720 Szeged, Hungary
来源：
PEPTIDES | 2002年 / 23卷 / 08期
关键词：
dwarf; rat; growth hormone-releasing hormone; receptor; binding; mRNA; anterior pituitary; somatotroph;
D O I：
10.1016/S0196-9781(02)00088-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Lewis dwarf (dw/dw) rats exhibit growth hormone (GH) deficiency and growth retardation linked to a malfunction of GHRH signaling. In this study, GHRH-receptor (GHRH-R) binding and mRNA in the pituitary of adult male dw/dw and age-matched normal Lewis rats was measured by radioligand binding assay and real-time PCR. Only one of nine pools of dw/dw pituitary membranes revealed detectable binding of [His(1), I-125-Tyr(10), Nle(27)]hGHRH(1-32) amide (B-max; 4.3 fmol/mg protein). In contrast, GHRH-R binding was 22.4 +/- 2.60 fmol/mg protein in normal Lewis rats. mRNA for GHRH-R was detectable in all dw/dw rat pituitaries examined, averaging 21% that of Lewis rats. Low expression of GHRH-R reflects reduced GHRH-R mRNA as well as a possible reduction in translation of the receptor protein. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：1497 / 1502
页数：6
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