Epithelial mesenchymal transition endows cells with migratory and invasive properties.Two groups of Hela cells (invasive group and non-invasive group) were retrieved utilizing Transwell Permeable Supports by Stromal cell-derived factor 1 alpha (SDF-1 alpha). In both the invasive and non-invasive groups, the morphological changes were observed under an inverted microscope, the expressions of mRNA and protein of E-cadherin and Vimentin were analyzed by RT-PCR assay and Western blot, and the levels of c were detected using RT-PCR. The authors found that compared with the non-invasive group, Hela cells in invasive group showed the mesenchymal morphologic changes, decreased expression of E-cadherin mRNA, and protein (p < 0.05), increased expression of Vimentin mRNA, and protein (p <0.05), up-regulated expression of Snail, Slug, and Twist mRNA (p <0.05). SDF-1 alpha was able to induce epithelial mesenchymal transition of Hela cells, and obtain the invasive features through up-regulation of Snail, Slug, and Twist.
机构:
Sichuan Univ, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China
Sichuan Univ, W China Hosp, State Key Lab Biotherapy, Div Stem Cell & Tissue Engn, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Dept Head & Neck Oncol, W China Coll Stomatol, Chengdu 610041, Sichuan, Peoples R China
Zhang, Fu-Gui
Yao, Yao
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机构:
Sichuan Univ, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China
Sichuan Univ, W China Hosp, State Key Lab Biotherapy, Div Stem Cell & Tissue Engn, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Dept Head & Neck Oncol, W China Coll Stomatol, Chengdu 610041, Sichuan, Peoples R China
Yao, Yao
Feng, Yang
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Sichuan Univ, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Dept Head & Neck Oncol, W China Coll Stomatol, Chengdu 610041, Sichuan, Peoples R China
Feng, Yang
Hua, Cheng-Ge
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Sichuan Univ, Dept Head & Neck Oncol, W China Coll Stomatol, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Dept Head & Neck Oncol, W China Coll Stomatol, Chengdu 610041, Sichuan, Peoples R China
Hua, Cheng-Ge
Tang, Xiu-Fa
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Sichuan Univ, Dept Head & Neck Oncol, W China Coll Stomatol, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Dept Head & Neck Oncol, W China Coll Stomatol, Chengdu 610041, Sichuan, Peoples R China
机构:
Charlie Norwood VA Med Ctr, Augusta, GA USA
Georgia Regents Univ, Dept Cellular Biol & Anat, Augusta, GA USACharlie Norwood VA Med Ctr, Augusta, GA USA
Herberg, Samuel
Shi, Xingming
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机构:
Georgia Regents Univ, Dept Pathol, Augusta, GA USA
Georgia Regents Univ, Inst Mol Med & Genet, Augusta, GA USA
Georgia Regents Univ, Inst Regenerat & Reparat Med, Augusta, GA USACharlie Norwood VA Med Ctr, Augusta, GA USA
Shi, Xingming
Johnson, Maribeth H.
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Georgia Regents Univ, Dept Biostat & Epidemiol, Augusta, GA USACharlie Norwood VA Med Ctr, Augusta, GA USA
Johnson, Maribeth H.
Hamrick, Mark W.
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机构:
Georgia Regents Univ, Dept Cellular Biol & Anat, Augusta, GA USA
Georgia Regents Univ, Dept Orthopaed Surg, Augusta, GA USA
Georgia Regents Univ, Inst Mol Med & Genet, Augusta, GA USA
Georgia Regents Univ, Inst Regenerat & Reparat Med, Augusta, GA USACharlie Norwood VA Med Ctr, Augusta, GA USA
Hamrick, Mark W.
Isales, Carlos M.
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Georgia Regents Univ, Dept Cellular Biol & Anat, Augusta, GA USA
Georgia Regents Univ, Dept Orthopaed Surg, Augusta, GA USA
Georgia Regents Univ, Inst Mol Med & Genet, Augusta, GA USA
Georgia Regents Univ, Inst Regenerat & Reparat Med, Augusta, GA USACharlie Norwood VA Med Ctr, Augusta, GA USA
Isales, Carlos M.
Hill, William D.
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机构:
Charlie Norwood VA Med Ctr, Augusta, GA USA
Georgia Regents Univ, Dept Cellular Biol & Anat, Augusta, GA USA
Georgia Regents Univ, Dept Orthopaed Surg, Augusta, GA USA
Georgia Regents Univ, Inst Mol Med & Genet, Augusta, GA USA
Georgia Regents Univ, Inst Regenerat & Reparat Med, Augusta, GA USACharlie Norwood VA Med Ctr, Augusta, GA USA
机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education
Junjie Wang
Weilin Yang
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机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education
Weilin Yang
Tao Wang
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机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education
Tao Wang
Xiaoyong Chen
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机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education
Xiaoyong Chen
Jiancheng Wang
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机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education
Jiancheng Wang
Xiaoran Zhang
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机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education
Xiaoran Zhang
Chuang Cai
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机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education
Chuang Cai
Beilong Zhong
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机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education
Beilong Zhong
Jiabin Wu
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机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education
Jiabin Wu
Zhenguang Chen
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机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education
Zhenguang Chen
Andy Peng Xiang
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机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education
Andy Peng Xiang
Weijun Huang
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机构:Sun Yat-Sen University,Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education