Stromal cell-derived factor 1α induce epithelial mesenchymal transition of Hela cells

被引:1
|
作者
Zhang, J. P. [1 ,2 ]
Feng, L. L. [3 ]
Zhan, H. F. [1 ,2 ]
Liu, Y. [1 ,2 ]
Wang, X. C. [4 ]
机构
[1] Jingan Dist Ctr Hosp Shanghai, Dept Gynecol, Shanghai, Peoples R China
[2] Fudan Univ, JingAn Branch, Huashan Hosp, 259 Xikang Rd, Shanghai 200040, Peoples R China
[3] Taizhou Second Hosp, Dept Obstet & Gynecol, Taizhou, Peoples R China
[4] Soochow Univ, Dept Anat, Coll Med, Suzhou, Peoples R China
关键词
Hela cell; Epithelial mesenchymal transition; E-cadherin; Vimentin; Snail; Twist; TRANSCRIPTION FACTORS SNAIL; E-CADHERIN EXPRESSION; REPRESSES E-CADHERIN; CANCER; TWIST; METASTASIS; SLUG; CARCINOMA; PROGRESSION; INVASION;
D O I
10.12892/ejgo3718.2017
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Epithelial mesenchymal transition endows cells with migratory and invasive properties.Two groups of Hela cells (invasive group and non-invasive group) were retrieved utilizing Transwell Permeable Supports by Stromal cell-derived factor 1 alpha (SDF-1 alpha). In both the invasive and non-invasive groups, the morphological changes were observed under an inverted microscope, the expressions of mRNA and protein of E-cadherin and Vimentin were analyzed by RT-PCR assay and Western blot, and the levels of c were detected using RT-PCR. The authors found that compared with the non-invasive group, Hela cells in invasive group showed the mesenchymal morphologic changes, decreased expression of E-cadherin mRNA, and protein (p < 0.05), increased expression of Vimentin mRNA, and protein (p <0.05), up-regulated expression of Snail, Slug, and Twist mRNA (p <0.05). SDF-1 alpha was able to induce epithelial mesenchymal transition of Hela cells, and obtain the invasive features through up-regulation of Snail, Slug, and Twist.
引用
收藏
页码:933 / 937
页数:5
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