Neural precursor cell expressed, developmentally clown-regulated gene 8 (NEDD8) is a recently discovered ubiquitin-like posttranslational modifier. NEDD8 acts predominantly as a regulator of ubiquitin-protein ligases and as a decoy for proteins targeted for proteasomal degradation. It thereby controls key events in cell cycle Progression and embryogenesis. Deneddylase-1 (DEN1/NEDP1/SENP8) features a selective peptidase activity converting the proNEDD8 precursor to its mature form and an isopeptidase activity deconjugating NEDD8 from substrates Such as cullins and p53. In this study, we describe a high-throughput screening (HTS)-compatible time-resolved fluorescent resonance energy transfer (TR-FRET) assay measuring the peptidase activity of DEN1. (C) 2009 Elsevier Inc. All rights reserved.
机构:
City Hope Natl Med Ctr, Beckman Res Inst, Dept Diabet Endocrinol & Metab, Duarte, CA 91010 USACity Hope Natl Med Ctr, Beckman Res Inst, Dept Diabet Endocrinol & Metab, Duarte, CA 91010 USA
Yu, Donna D.
Lin, Wenwei
论文数: 0引用数: 0
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机构:
St Jude Childrens Res Hosp, Dept Chem Biol & Therapeut, Memphis, TN 38105 USACity Hope Natl Med Ctr, Beckman Res Inst, Dept Diabet Endocrinol & Metab, Duarte, CA 91010 USA
Lin, Wenwei
Chen, Taosheng
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St Jude Childrens Res Hosp, Dept Chem Biol & Therapeut, Memphis, TN 38105 USACity Hope Natl Med Ctr, Beckman Res Inst, Dept Diabet Endocrinol & Metab, Duarte, CA 91010 USA
Chen, Taosheng
Forman, Barry M.
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City Hope Natl Med Ctr, Beckman Res Inst, Dept Diabet Endocrinol & Metab, Duarte, CA 91010 USACity Hope Natl Med Ctr, Beckman Res Inst, Dept Diabet Endocrinol & Metab, Duarte, CA 91010 USA
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Univ Pittsburgh, Dept Psychiat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Psychiat, Pittsburgh, PA 15260 USA
Aslanoglou, Despoina
George, Emily W.
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Univ Pittsburgh, Dept Psychiat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Psychiat, Pittsburgh, PA 15260 USA
George, Emily W.
Freyberg, Zachary
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机构:
Univ Pittsburgh, Dept Psychiat, Pittsburgh, PA 15260 USA
Univ Pittsburgh, Dept Cell Biol, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Psychiat, Pittsburgh, PA 15260 USA
Freyberg, Zachary
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Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R ChinaChinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
Xu, Zhengping
Zhou, Hong
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机构:
Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R ChinaChinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
Zhou, Hong
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Li, Li
Chen, Zhang
论文数: 0引用数: 0
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机构:
Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R ChinaChinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
Chen, Zhang
Zhang, Xin
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Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R ChinaChinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
Zhang, Xin
Feng, Yongtong
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Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R ChinaChinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
Feng, Yongtong
Wang, Jianping
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Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R ChinaChinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
Wang, Jianping
Li, Yuan
论文数: 0引用数: 0
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机构:
Chongqing Guoke Med Innovat Technol Dev Co Ltd, Chongqing 401122, Peoples R ChinaChinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
Li, Yuan
Wu, Yanfan
论文数: 0引用数: 0
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机构:
Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R ChinaChinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
机构:
Univ Helsinki, Fac Med, Dept Virol, Medicum, Helsinki 00290, FinlandUniv Helsinki, Fac Med, Dept Virol, Medicum, Helsinki 00290, Finland
Lintala, Annika
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Vapalahti, Olli
Nousiainen, Arttu
论文数: 0引用数: 0
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机构:
Univ Helsinki, Fac Med, Human Microbiome Res Program, Helsinki 00014, Finland
Univ Helsinki, Meilahti Infect Dis & Vaccine Res Ctr, Dept Infect Dis, MeiVac, Helsinki 00029, Finland
Helsinki Univ Hosp, Helsinki 00029, FinlandUniv Helsinki, Fac Med, Dept Virol, Medicum, Helsinki 00290, Finland
Nousiainen, Arttu
论文数: 引用数:
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Kantele, Anu
Hepojoki, Jussi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Helsinki, Fac Med, Dept Virol, Medicum, Helsinki 00290, Finland
Univ Zurich, Inst Vet Pathol, Vetsuisse Fac, CH-8057 Zurich, SwitzerlandUniv Helsinki, Fac Med, Dept Virol, Medicum, Helsinki 00290, Finland