In vitro binding of nucleolin to double-stranded telomeric DNA

被引：18
|
作者：
Pollice, A
Zibella, MP
Bilaud, T
Laroche, T
Pulitzer, JF
Gilson, E
机构：
[1] Univ Naples Federico II, Dipartimento Genet Biol Gen & Mol, I-80134 Naples, Italy
[2] Int Inst Genet & Biophys, I-80100 Naples, Italy
[3] Swiss Inst Expt Canc Res, CH-1066 Epalinges, Switzerland
[4] Ecole Normale Super Lyon, CNRS, Biol Cellulaire & Mol Lab, UMR5665, F-69364 Lyon 07, France
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 268卷 / 03期
关键词：
telomere; nucleolin; DNA-binding; mammalian;
D O I：
10.1006/bbrc.2000.2237
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have purified a 100 kDa protein, resolved in a Southwestern binding screen of total nuclear proteins from Hela cells with double-stranded human telomeric probe. A polyclonal antiserum raised by this protein recognizes purified nucleolin and stains nucleoli in growing Hela cells. We demonstrate that a truncated form of human nucleolin and a purified deletion derivative of mouse nucleolin bind in vitro to duplex telomeric DNA. This study suggests a new link between telomeres and the nucleolus. (C) 2000 Academic Press.
引用
收藏
页码：909 / 915
页数：7
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