An evaluation of the effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-intoxicated rabbits

Objectives: Lead exposure has destructive effects on some organs. It may produce a variety of toxic effects on endothelial cells of the vascular system. Any changes or damages to endothelial cells may lead to cardiovascular diseases, particularly the formation of atherosclerotic plaques. The aim of this study was to determine the ameliorative effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-exposed rabbits. Methods: In this study, 30 white male rabbits of New Zealand race (weighing about 1.6-2 kg and 5 months old) were used and divided randomly into three groups: Group 1 (N = 10) that served as the control and received water and normal diet, Group 2 (N = 10) was exposed to lead acetate 547 ppm (5 mg/L) daily for 40 days, and Group 3 (N = 10) received vitamin C (500 mg/kg) and underwent the same duration of lead exposure (5 mg/L) daily for 40 days. The levels of cholesterol, triglyceride, low-density lipoprotein, and high-density lipoprotein were measured using spectrophotometry, and the level of blood lead was calculated using a lead analyzer (Magellan Diagnostics, USA). The animals were anesthetized by pentobarbital (50 mg/kg). Subsequently, they were sacrificed, and their thoracic aortas and coronary arteries were removed. Then fixation, tissue processing, histological sectioning, and H & E staining were carried out. Finally, the sections were studied using light microscopy. The results were analyzed using the Mann-Whitney test. Results: The results indicated that ascorbic acid could reduce the destructive effects of lead on vascular endothelial cells and prevent the formation of atherosclerotic plaques in coronary and aorta arteries. Conclusion: The results of this study confirm the beneficial effects of ascorbic acid against the destructive effects of lead on vascular endothelial cells. Hence, it could be proposed as a potential prophylactic treatment for the amelioration of lead toxicity, prevention of atherosclerosis, and improvement of endothelial cells dysfunction.