Comparative structural analysis of fruit and stem bromelain from Ananas comosus

被引:29
|
作者
Ramli, Aizi Nor Mazila [1 ]
Manas, Nor Hasmaliana Abdul [2 ]
Hamid, Azzmer Azzar Abdul [3 ]
Abd Hamid, Hazrulrizawati [1 ]
Illias, Rosli Md. [2 ]
机构
[1] Univ Malaysia Pahang, Fac Ind Sci & Technol, Kuantan 26300, Pahang Darul Ma, Malaysia
[2] Univ Teknol Malaysia, Fac Chem & Energy Engn, Dept Bioproc & Polymer Engn, Skudai 81310, Johor, Malaysia
[3] Int Islamic Univ Malaysia, Kulliyyah Sci, Dept Biotechnol, Jalan Sultan Ahmad Shah, Kuantan 25200, Pahang, Malaysia
关键词
Stem bromelain; Fruit bromelain; Amino acid analysis; 3D structure prediction; GLACIOZYMA-ANTARCTICA PI12; AMINO-ACID-SEQUENCE; CYSTEINE PROTEINASES; PINEAPPLE FRUIT; PREDICTION; PURIFICATION; CARBOHYDRATE; STABILITY; MECHANISM; PROTEASES;
D O I
10.1016/j.foodchem.2018.05.125
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Cysteine proteases in pineapple (Ananas comosus) plants are phytotherapeutical agents that demonstrate antiedematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities. Bromelain has been identified as an active component and as a major protease of A. comosus. Bromelain has gained wide acceptance and compliance as a phytotherapeutical drug. The proteolytic fraction of pineapple stem is termed stem bromelain, while the one presents in the fruit is known as fruit bromelain. The amino acid sequence and domain analysis of the fruit and stem bromelains demonstrated several differences and similarities of these cysteine protease family members. In addition, analysis of the modelled fruit (BAA21848) and stem (CAA08861) bromelains revealed the presence of unique properties of the predicted structures. Sequence analysis and structural prediction of stem and fruit bromelains of A. comosus along with the comparison of both structures provides a new insight on their distinct properties for industrial application.
引用
收藏
页码:183 / 191
页数:9
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