A sensitive enzyme-linked immunosorbent assay amplified by biotin-streptavidin system for detecting non-steroidal anti-inflammatory drug ketoprofen

被引：2
|
作者：
Bu, Dan [1 ]
Zhuang, Hui S. [1 ]
Yang, Guang X. [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Environm Sci & Technol, Inst Environm Monitoring & Evaluat, Shanghai 200030, Peoples R China
来源：
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES | 2014年 / 49卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Biotin-streptavidin; ELISA; fish samples; ketoprofen; PPCPs; PERFORMANCE LIQUID-CHROMATOGRAPHY; ELECTRON-CAPTURE DETECTOR; TANDEM MASS-SPECTROMETRY; CHLORAMPHENICOL RESIDUES; GAS-CHROMATOGRAPHY; FLORFENICOL AMINE; IMMUNOASSAY; THIAMPHENICOL; TISSUES; ELISA;
D O I：
10.1080/03601234.2014.894776
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A sensitive biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) method was developed for detecting non-steroidal anti-inflammatory drug ketoprofen. Compared with traditional ELISA method, the sensitivity of proposed immunoassay was enhanced by the biotin-streptavidin system. Under the optimal condition, the median inhibitory concentration (IC50) was 0.25ng mL(-1), with minor cross-reactivity to a number of structural analogs. This developed assay was successfully applied to detect the ketoprofen residues in different fish samples, and good recoveries (72.6-105.5%) were obtained. The results indicated that this immunoassay method could specifically detect trace ketoprofen residues and could be widely used for routine monitoring of food samples.
引用
收藏
页码：408 / 415
页数：8
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