Graphene coating on the surface of CoCrMo alloy enhances the adhesion and proliferation of bone marrow mesenchymal stem cells

被引：17
|
作者：
Zhang, Qi [1 ,2 ]
Li, Kewen [3 ]
Yan, Jinhong [1 ]
Wang, Zhuo [1 ]
Wu, Qi [1 ]
Bi, Long [1 ]
Yang, Min [1 ]
Han, Yisheng [1 ]
机构：
[1] Fourth Mil Med Univ, Xijing Hosp, Dept Orthoped, 15 Changle West Rd, Xian 710032, Shaanxi, Peoples R China
[2] 323 Hosp Chinese PM, Dept Orthoped, Xian, Shaanxi, Peoples R China
[3] Qinghai Univ, Affiliated Hosp, Dept Orthoped, Xining, Qinghai, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 497卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Graphene; CoCrMo alloy; Wet transfer; Bone marrow mesenchymal stem cells; Bioactivity; NANO-GRAPHENE; DIFFERENTIATION;
D O I：
10.1016/j.bbrc.2018.02.152
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The objective was to investigate whether a graphene coating could improve the surface bioactivity of a cobalt-chromium-molybdenum-based alloy (CoCrMo). Graphene was produced by chemical vapor deposition and transferred to the surface of the CoCrMo alloy using an improved wet transfer approach. The morphology of the samples was observed, and the adhesion force and stabilization of graphene coating were analyzed by a nanoscratch test and ultrasonication test. In an in vitro study, the adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) cultured on the samples were quantified via an Alamar Blue assay and cell counting kit-8 (CCK-8) assay. The results showed that it is feasible to apply graphene to modify the surface of a CoCrMo alloy, and the enhancement of the adhesion and proliferation of BMSCs was also shown in the present study. In conclusion, graphene exhibits considerable potential for enhancing the surface bioactivity of CoCrMo alloy. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码：1011 / 1017
页数：7
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