Post-transcriptional regulation of the xynA expression by a novel mRNA binding protein, XaiF

XaiF, a novel 32 kDa protein encoded by the ORF located in the immediate downstream of the xynA gene of Bacillus stearotherniophilus No. 236, was identified to be the xylanase-specific trans-activator. In this study, the positive effect of XaiF was confirmed to be xylanase-specific, and the results from Northern blot and in vitro transcription assays showed that the XaiF increased the xynA transcripts at post-transcriptional step. Moreover, analysis of the mRNA decay rate led to the assertion that the XaiF functions to stabilize the xynA mRNA. Intriguingly, in vitro RNA-protein binding assay and analysis using gst-xynA 3'-UTR chimeric gene constructs demonstrated that the XaiF stabilizes xynA mRNA by direct binding onto the 3'-UTR of the mRNA. (c) 2006 Elsevier Inc. All rights reserved.