Evaluation of a rapid immunochromatographic assay for the detection of Legionella antigen in urine samples

被引：56
|
作者：
Domínguez, J [1 ]
Galí, N [1 ]
Matas, L [1 ]
Pedroso, P [1 ]
Hernández, A [1 ]
Padilla, E [1 ]
Ausina, V [1 ]
机构：
[1] Univ Autonoma Barcelona, Hosp Univ Germans Trias Pujol, Microbiol Serv, Fac Med, E-08916 Barcelona, Spain
来源：
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 1999年 / 18卷 / 12期
关键词：
D O I：
10.1007/s100960050427
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A new immunochromatographic membrane assay for detecting Legionella pneumophila serogroup 1 antigen in urine samples (Binax Now Legionella Urinary Antigen Test; Binax, USA) was evaluated. Its sensitivity, specificity and level of agreement with the Binax enzyme immunoassay were compared using nonconcentrated and concentrated urine samples. The overall agreement between the two tests was 98.1%: the specificity of both was 100%. The sensitivity of the immunochromatographic assay was 55.5% in nonconcentrated urine and 97.2% in concentrated urine in comparison with the enzyme immunoassay, using concentrated urine as the reference test. This immunochromatographic assay screens successfully for Legionella pneumophila serogroup 1 soluble antigen in concentrated urine samples.
引用
收藏
页码：896 / 898
页数：3
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