Multiplexed Sandwich Immunoassays Using Electrochemiluminescence Imaging Resolved at the Single Bead Level

被引：209
|
作者：
Deiss, Frederique [1 ]
LaFratta, Christopher N. [2 ]
Symer, Matthew [2 ]
Blicharz, Timothy M. [2 ]
Sojic, Neso [1 ]
Walt, David R. [2 ]
机构：
[1] Univ Bordeaux, Inst Mol Sci, ENSCPB, F-33607 Pessac, France
[2] Tufts Univ, Dept Chem, Medford, MA 02155 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 17期
基金：
美国国家科学基金会;
关键词：
ELECTROGENERATED CHEMILUMINESCENCE; AMPLIFICATION; ARRAYS; SYSTEM;
D O I：
10.1021/ja901876z
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A new class of bead-based microarray that uses electrogenerated chemiluminescence (ECL) as a readout mechanism to detect multiple antigens simultaneously is presented. This platform demonstrates the possibility of performing highly multiplexed assays using ECL because all the individual sensing beads in the array are simultaneously imaged and individually resolved by ECL. Duplex and triplex assay results are demonstrated as well as a cross reactivity study.
引用
收藏
页码：6088 / +
页数：3
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