Production of recombinant rotavirus capsid protein VP7 from stably transformed Drosophila melanogaster S2 cells

被引：0

作者：

Park, JH

Chang, KH

Lee, YH

Kim, HY

Yang, JM

Chung, IS ^{[1
]}

机构：

[1] Kyung Hee Univ, Dept Genet Engn, Suwon 449701, South Korea

[2] Kyung Hee Univ, Plant Metab Res Ctr, Suwon 449701, South Korea

[3] Kyung Hee Univ, Grad Sch Biotechnol, Suwon 449701, South Korea

[4] Sogang Univ, Dept Life Sci, Seoul 121742, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2002年 / 12卷 / 04期

关键词：

Drosophila melanogaster S2 cell; rotavirus capsid protein VP7; DMSO; sodium butyrate;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Stably transformed Drosophila melanogaster S2 cells producing recombinant VP7 were obtained, and recombinant VP7 expression was confirmed by Western blot analysis. The molecular weight of recombinant VP7 expressed in S2 cells was approximately 35.5 kDa, and 75% of the total VP7 produced was present in the medium. Recombinant VP7 contained N-linked glycosylated oligosaccharides. Aprotinin, leupeptin, and polyvinylpyrrolidone did not have any noticeable effect on recombinant VP7 production; however, DMSO and sodium butyrate increased its production by 120% and 60%, respectively.

引用

页码：563 / 568

页数：6

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