The fraction of DNA released on pulsed-field gel electrophoresis gels may differ significantly between genomes at low levels of double-strand breaks

被引：0
|
作者：
Cedervall, BE
McMillan, TJ
机构：
[1] Karolinska Inst, Dept Pathol & Oncol, SE-17176 Stockholm, Sweden
[2] Univ Lancaster, Dept Biol Sci, Inst Environm & Mat Sci, Lancaster LA1 4YQ, England
来源：
RADIATION RESEARCH | 2002年 / 158卷 / 02期
关键词：
D O I：
10.1667/0033-7587(2002)158[0247:TFODRO]2.0.CO;2
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A common way to use pulsed-field gel electrophoresis (PFGE) for measuring the induction and repair of DNA double-strand breaks (DSBs) in mammalian cells is by using the fraction of total DNA released, FR, from the plug. We have analyzed the general relationship between initial chromosome sizes and FR. It is shown that, because of the difference in initial chromosomal size, the discrepancy in FR values between human and rodent cells may become significant at doses of radiation producing similar to5 DSBs/100 Mbp or less. (C) 2002 by Radiation Research Society.
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页码：247 / 249
页数：3
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