Resveratrol binding to human serum albumin

被引:73
|
作者
N'soukpoe-Kossi, C. N.
St-Louis, C.
Beauregard, M.
Subirade, M.
Carpentier, R.
Hotchandani, S.
Tajmir-Riahi, H. A.
机构
[1] Univ Quebec Trois Rivieres, Dept Chim Biol, Trois Rivieres, PQ G9A 5H7, Canada
[2] Univ Laval, Inst Rech Nutraceut & Aliments Fonct, INAF STELA, Ste Foy, PQ G1K 7P4, Canada
来源
关键词
resveratrol; protein; binding mode; binding constant; secondary structure; FTIR; UV-Vis; CD; and fluorescence spectroscopy;
D O I
10.1080/07391102.2006.10507120
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Resveratrol (Res), a polyphenolic compound found largely in the skin of red grape and wine, exhibits a wide range of pharmaceutical properties and plays a role in prevention of human cardiovascular diseases [Pendurthi et al., Arterioscler. Thromb. Vasc. Biol. 19, 419-426 (1999)]. It shows a strong affinity towards protein binding and used as inhibitor for cyclooxygenase and ribonuclease reductase. The aim of this study was to examine the interaction of resveratrol with human serum albumin (HSA) in aqueous solution at physiological conditions, using a constant protein concentration (0.3 mM) and various pigment contents (mu M to mM). FTIR, UV-Visible, CD, and fluorescence spectroscopic methods were used to determine the resveratrol binding mode, the binding constant and the effects of pigment complexation on protein secondary structure. Structural analysis showed that resveratrol bind non-specifically (H-bonding) via polypeptide polar groups with overall binding constant of K-Res = 2.56 x 10(5) M-1. The protein secondary structure, analysed by CD spectroscopy, showed no major alterations at low resveratrol concentrations (0.125 mM), whereas at high pigment content (I mM), major increase of cc-helix from 57% (free HSA) to 62% and a decrease of beta-sheet from 10% (free HSA) to 7% occurred in the resveratrol-HSA complexes. The results indicate a partial stabilization of protein secondary structure at high resveratrol content.
引用
收藏
页码:277 / 283
页数:7
相关论文
共 50 条
  • [21] BINDING OF CHLORPROMAZINE TO HUMAN-SERUM ALBUMIN
    SHARPLES, D
    JOURNAL OF PHARMACY AND PHARMACOLOGY, 1974, 26 (08) : 640 - 641
  • [22] BINDING OF DIPHENYLHYDANTOIN BY HUMAN SERUM-ALBUMIN
    BROWNE, RK
    ZAROSLIN.JF
    OESTER, TY
    FEDERATION PROCEEDINGS, 1972, 31 (02) : A537 - &
  • [23] Binding of tolbutamide to glycated human serum albumin
    Joseph, K. S.
    Anguizola, Jeanethe
    Hage, David S.
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2011, 54 (02) : 426 - 432
  • [24] BINDING OF PROSTAGLANDIN TO HUMAN SERUM-ALBUMIN
    UNGER, WG
    JOURNAL OF PHARMACY AND PHARMACOLOGY, 1972, 24 (06) : 470 - &
  • [25] Binding of disodium cromoglycate to human serum albumin
    de Aspuru, EO
    Zaton, AML
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 1998, 54 (07) : 983 - 988
  • [26] Binding of human serum albumin to various medications
    Manyanga, Fidelis
    Calnan, Angelina
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 256
  • [27] BINDING OF BROMOCRESOL GREEN BY HUMAN SERUM ALBUMIN
    RODKEY, FL
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1964, 108 (03) : 510 - &
  • [28] Binding of ochratoxin A derivatives to human serum albumin
    Perry, JL
    Il'ichev, YV
    Kempf, VR
    McClendon, J
    Park, G
    Manderville, RA
    Rüker, F
    Dockal, M
    Simon, JD
    JOURNAL OF PHYSICAL CHEMISTRY B, 2003, 107 (27): : 6644 - 6647
  • [29] Cooperative binding of drugs on human serum albumin
    Varela, LM
    Pérez-Rodríguez, M
    García, M
    MOLECULAR PHYSICS, 2004, 102 (01) : 79 - 84
  • [30] BINDING OF CATECHOLAMINES TO HUMAN SERUM-ALBUMIN
    DANON, A
    SAPIRA, JD
    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 1972, 182 (02): : 295 - &