Development and Application of SYBR Green I Real-Time Fluorescence PCR for Detection of Porcine Epidemic Diarrhea Virus

被引：0

作者：

Guo, Rui ^{[1
]}

Tian, Yongxiang ^{[1
]}

Zhou, Danna ^{[1
]}

Duan, Zhengying ^{[1
]}

Yang, Keli ^{[1
]}

Liu, Zewen ^{[1
]}

Yuan, Fangyan ^{[1
]}

机构：

[1] Hubei Acad Agr Sci, Inst Anim Husb & Vet, Wuhan 430064, Hunan, Peoples R China

来源：

2014 INTERNATIONAL CONFERENCE ON BIOLOGICAL ENGINEERING AND BIOMEDICAL (BEAB 2014) | 2014年

关键词：

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

With the conservative gene sequence ORF3 of porcine epidemic diarrhea virus (PEDV) as reference, a pair of specific PCR primers were designed and optimized. Then SYBR Green I real-time fluorescence PCR was developed for rapid quantitative detection of PEDV. This method has a sensibility 100 times higher than RT-PCR and avoids the high pollution brought by conventional PCR electrophoresis. Therefore, with high speediness, sensibility, specificity, repeatability, and quantifying ability, this method can be used in rapid detection of PEDV in pig farms.

引用

页码：281 / 287

页数：7

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