Viability of Campylobacter spp. in frozen and chilled broiler carcasses according to real-time PCR with propidium monoazide pretreatment

被引：19
|
作者：
Castro, Andrea G. S. A. [1 ]
Dorneles, Elaine M. S. [2 ]
Santos, Ethiene L. S. [1 ]
Alves, Telma M. [3 ]
Silva, Guilherme R. [1 ]
Figueiredo, Tadeu C. [1 ]
Assis, Debora C. S. [1 ]
Lage, Andrey P. [3 ]
Cancado, Silvana, V [1 ]
机构：
[1] Univ Fed Minas Gerais, Escola Vet, Dept Tecnol & Inspecao Prod Origem Anim, Av Antonio Carlos 6627,Caixa Postal 567, BR-30123970 Belo Horizonte, MG, Brazil
[2] Univ Fed Lavras, Dept Med Vet, Av Dr Sylvio Menicucci 1001,Caixa Postal 3037, BR-37200000 Lavras, MG, Brazil
[3] Univ Fed Minas Gerais, Escola Vet, Dept Med Vet Prevent, Av Antonio Carlos 6627,Caixa Postal 567, BR-30123970 Belo Horizonte, MG, Brazil
来源：
POULTRY SCIENCE | 2018年 / 97卷 / 05期
关键词：
chicken meat; foodborne disease; DNA intercalator; campylobacteriosis; CHICKEN CARCASSES; RISK-FACTORS; JEJUNI; PREVALENCE; SKIN; DIFFERENTIATION; QUANTIFICATION; CONTAMINATION; TEMPERATURE; SALMONELLA;
D O I：
10.3382/ps/pey020
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The aim of this study was to evaluate the viability of Campylobacter spp. in frozen and chilled broiler carcasses using real-time PCR with propidium monoazide (PMA) pretreatment. Sixty broiler carcasses were collected: 30 frozen and 30 chilled. Each carcass was submitted to 2 real-time PCR protocols to detect and quantify Campylobacter spp.: one using pretreatment with PMA, which blocks the amplification of DNA from dead bacteria, and the other without PMA. The results showed that PMA-pretreated carcasses, either frozen or chilled, had a lower positivity rate compared to untreated samples (P < 0.001). Regarding storage temperatures, PMA-pretreated frozen carcasses that tested positive were in a lesser number than chilled carcasses (P < 0.05). However, the quantification of total and live bacteria in PMA-pretreated frozen carcasses that tested positive showed no significant difference compared to chilled carcasses. It was concluded that the real-time PCR with PMA pretreatment was a sensitive method for evaluating the viability of Campylobacter spp. in broiler carcasses. Chilled broiler carcasses would represent greater hazard to public health concerning Campylobacter transmission.
引用
收藏
页码：1706 / 1711
页数：6
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