Comprehensive two-dimensional high-performance liquid chromatography system with immobilized liposome chromatography column and reversed-phase column for separation of complex traditional Chinese medicine Longdan Xiegan Decoction

被引：66
|
作者：
Wang, Yun [1 ,2 ,3 ]
Kong, Liang [4 ]
Lei, Xiaoyuan [1 ]
Hu, Lianghai [1 ]
Zou, Hanfa [1 ]
Welbeck, Ed [5 ]
Bligh, S. W. Annie [5 ]
Wang, Zhengtao [2 ,3 ]
机构：
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
[2] Shanghai Univ Tradit Chinese Med, Minist Educat, Key Lab Standuidizat Chinese Med, Shanghai 201210, Peoples R China
[3] China Pharmaceut Univ, Dept Pharmacognosy, Nanjing 210038, Peoples R China
[4] Dalian Fisheries Univ, Coll Marine Environm Engn, Dalian 116023, Peoples R China
[5] London Metropolitan Univ, Inst Hlth Res & Policy, London N7 8D8, England
来源：
JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 11期
基金：
中国国家自然科学基金;
关键词：
Comprehensive two-dimensional chromatography; ILC column; Biological finger printing analysis; Longdan Xiegan Decoction; Traditional Chinese medicine; TANDEM MASS-SPECTROMETRY; PROTEIN; IDENTIFICATION; MEMBRANE; HPLC; PRESCRIPTION; DIGESTION;
D O I：
10.1016/j.chroma.2008.05.074
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A comprehensive two-dimensional HPLC system with an immobilized liposome chromatography (ILC) column in conjunction with all RP column (in tandem) was developed for the screening and analysis of the membrane-permeable Compounds in a traditional Chinese medicine prescription Longdan Xiegan Decoction (LXD). More than 50 components in LXD were resolved using the developed separation system. Eight flavonoids and two iridoids were identified interacting with the ILC column: a system that mimics biomembranes. The results show that the developed comprehensive two-dimensional chromatography system can be used for identifying membrane permeable natural products in complex matrixes such as extracts of traditional Chinese medicine prescriptions. (C) 2008 Published by Elsevier B.V.
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页码：2185 / 2191
页数：7
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