In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation

被引：13
|
作者：
Choi, Hyun Woo [1 ,2 ]
Hong, Yean Ju [1 ]
Kim, Jong Soo [1 ]
Song, Hyuk [1 ]
Cho, Ssang Gu [1 ]
Bae, Hojae [3 ]
Kim, Changsung [4 ]
Byun, Sung June [5 ]
Do, Jeong Tae [1 ]
机构：
[1] Konkuk Univ, Coll Anim Biosci & Technol, Dept Anim Biotechnol, Seoul, South Korea
[2] Max Planck Inst Mol Biomed, Dept Cell & Dev Biol, Munster, Germany
[3] Konkuk Univ, Coll Anim Biosci & Technol, Dept Bioind Technol, Seoul, South Korea
[4] Sejong Univ, Dept Biosci & Biotechnol, Seoul, South Korea
[5] RDA, Natl Inst Anim Sci, Anim Biotechnol Div, Wanju Gun, Jeollabuk Do, South Korea
来源：
PLOS ONE | 2017年 / 12卷 / 01期
基金：
新加坡国家研究基金会;
关键词：
TETRAPLOID MOUSE EMBRYOS; CENTRAL-NERVOUS-SYSTEM; TERATOMA FORMATION; SELF-RENEWAL; GENERATION; AGGREGATION; INDUCTION; NEURONS; CULTURE; NICHE;
D O I：
10.1371/journal.pone.0170735
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Like embryonic stem cells, induced pluripotent stem cells (iPSCs) can differentiate into all three germ layers in an in vitro system. Here, we developed a new technology for obtaining neural stem cells (NSCs) from iPSCs through chimera formation, in an in vivo environment. iPSCs contributed to the neural lineage in the chimera, which could be efficiently purified and directly cultured as NSCs in vitro. The iPSC-derived, in vivo-differentiated NSCs expressed NSC markers, and their gene-expression pattern more closely resembled that of fetal brain-derived NSCs than in vitro-differentiated NSCs. This system could be applied for differentiating pluripotent stem cells into specialized cell types whose differentiation protocols are not well established.
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页数：13
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