Detection and Identification of Sarcocystis cruzi (Protozoa: Apicomplexa) by Molecular and Ultrastructural Studies in Naturally Infected Korean Cattle (Bos taurus coreanae) from Daejeon, Korea

被引:11
|
作者
Choi, Tong-Il [1 ]
Hong, Eui-Ju [1 ]
Ryu, Si-Yun [1 ]
Sim, Cheolho [2 ]
Chae, Joon-Seok [3 ,4 ]
Kim, Hyeon-Cheol [5 ]
Park, Jinho [6 ]
Choi, Kyoung-Seong [7 ]
Yu, Do-Hyeon [8 ]
Yoo, Jae-Gyu [9 ]
Park, Bae-Keun [1 ]
机构
[1] Chungnam Natl Univ, Coll Vet Med, Daejeon 34134, South Korea
[2] Baylor Univ, Dept Biol, Waco, TX 76798 USA
[3] Seoul Natl Univ, Lab Vet Internal Med, PLUS Program Creat Vet Sci Res BK21, Seoul 08826, South Korea
[4] Seoul Natl Univ, Coll Vet Med, Seoul 08826, South Korea
[5] Kangwon Natl Univ, Coll Vet Med, Chunchon 24341, South Korea
[6] Chonbuk Natl Univ, Coll Vet Med, Jeonju 54896, South Korea
[7] Kyungpook Natl Univ, Coll Anim Sci, Daegu 41566, South Korea
[8] Gyeongsang Natl Univ, Coll Vet Med, Jinju 52828, South Korea
[9] Natl Inst Anim Sci Rural Dev Adm, Lab Vet Clin, Wonju 55365, South Korea
来源
KOREAN JOURNAL OF PARASITOLOGY | 2018年 / 56卷 / 02期
关键词
Sarcocystis cruzi; Bos taurus coreanae; ultrastructure; 18S rRNA; WATER-BUFFALO; SPOROCYSTS; CALVES; CYSTS;
D O I
10.3347/kjp.2018.56.2.121
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
To survey the prevalence of Sarcocystis infections, 210 heart samples were collected from Korean native cattle (Bos taurus coreanae) at an abattoir in Daejeon Metropolitan City, Republic of Korea. Sarcocysts were detected form 31 specimens (14.8%) and identified as Sarcocystis cruzi via transmission electron microscopy. The wall of S. cruzi has flattened protrusions that did not contain fibrils or microfilaments. The protrusions arose irregularly from the base, contained a fine granular substance, lacked internal microfilaments, and measured approximately 0.21 - 1.25 mu m in length and 0.05-0.07 mu m in width. Sequence analysis revealed 99.5% homology to S. cruzi. This is the first report on the prevalence of S. cruzi in native cattle from the Republic of Korea.
引用
收藏
页码:121 / 127
页数:7
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