Isolation and biological and molecular characterization of Neospora caninum (NC-SP1) from a naturally infected adult asymptomatic cattle (Bos taurus) in the state of Sao Paulo, Brazil

被引：9

作者：

Oliveira, Solange ^{[1
]}

Soares, Rodrigo Martins ^{[1
]}

Aizawa, Juliana ^{[1
]}

Soares, Herbert Sousa ^{[1
]}

Chiebao, Daniela Pontes ^{[1
]}

Miguel Ortega-Mora, Luis ^{[2
]}

Regidor-Cerrillo, Javier ^{[2
]}

Bressa Silva, Natalia Quadros ^{[1
]}

Gennari, Solange Maria ^{[1
]}

Jesus Pena, Hilda Fatima ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Vet Med, Dept Prevent Vet Med & Anim Hlth, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, Brazil

[2] Univ Complutense Madrid, Fac Vet Sci, AnimalHlth Dept, SALUVET, Ciudad Univ S-N, Madrid 28040, Spain

来源：

PARASITOLOGY | 2017年 / 144卷 / 06期

基金：

巴西圣保罗研究基金会;

关键词：

Neosporosis; cattle; bioassay; oocyst shedding; gerbils; microsatellite analysis; TOXOPLASMA-GONDII; HAMMONDIA-HEYDORNI; GENETIC DIVERSITY; OOCYSTS; DOGS; PCR; HOST; PATTERNS; TISSUES;

D O I：

10.1017/S0031182016002481

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The biological and genetic diversity of Neospora caninum is very limited because of availability of only a few viable isolates worldwide. This study describes the isolation and biological and molecular characterization of a new viable isolate of N. caninum (NC-SP1), from a cattle in Brazil. Approximately 400 g of brain from a naturally infected adult male cattle from an abattoir was fed to a 2-month-old dog. Neospora-like oocysts were observed on day 7 post-inoculation (PI) and the duration of oocyst shedding was 14 days. The DNA obtained from oocysts was characterized molecularly and the final sequence was 99% identical to homologous sequences of N. caninum available in GenBank (R). For bioassay, gerbils (Meriones unguiculatus) were orally inoculated with 10 100 and 1000 oocysts; all gerbils remained clinically normal but developed N. caninum antibodies 14 days PI. Cell culture isolation was successful using the brain homogenate from one of the gerbils and tachyzoites were observed 24 days PI. Microsatellite genotyping revealed a unique genetic profile for this new reference isolate.

引用

页码：707 / 711

页数：5

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