Immunolocalisation of myosin-V in the enteric nervous system of the rat

被引：26
|
作者：
Drengk, AC
Kajiwara, JK
Garcia, SB
Carmo, VS
Larson, RE
Zucoloto, S
Espreafico, EM
机构：
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Morphol, BR-14049900 Ribeirao Preto, SP, Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biochem, Ribeirao Preto, Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Pathol, Ribeirao Preto, Brazil
[4] Imperial Canc Res Fund, London WC2A 3PX, England
来源：
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM | 2000年 / 78卷 / 2-3期
基金：
巴西圣保罗研究基金会;
关键词：
myosin-V; enteric nervous system; immunohistochemistry;
D O I：
10.1016/S0165-1838(99)00073-9
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We show here the localisation of myosin-V in whole mount preparations of the mucous-submucous and the muscular layers of rat small intestine by using an affinity purified antibody specific to the tail domain of myosin-V. Myosin-V immunostaining was intense in the submucous and myenteric nervous plexuses, allowing the visualisation of neuronal cell bodies and fibres. Western blots of total muscle layers homogenates detected with the same antibody revealed a single band of the expected size for myosin-V. Understanding the cellular localisation and function of this class of myosin is an important challenge and the accessibility and simplicity of the enteric nervous system as compared to the central nervous system, makes the digestive tract an attractive model for studying possible functional roles of myosin-V in neurotransmission and neuroplasticity. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：109 / 112
页数：4
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