A method of screening for highly inhibitory lactic acid bacteria

被引：13
|
作者：
Kociubinski, GL [1 ]
Perez, PF [1 ]
Anon, MC [1 ]
DeAntoni, GL [1 ]
机构：
[1] CTR INVEST & DESARROLLO CRIOTECNOL ALIMENTOS,RA-1900 LA PLATA,ARGENTINA
来源：
JOURNAL OF FOOD PROTECTION | 1996年 / 59卷 / 07期
关键词：
lactic acid bacteria; inhibition; agar-diffusion assay; pH; Lactobacillus; Streptococcus; Bifidobacterium;
D O I：
10.4315/0362-028X-59.7.739
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A rapid method of screening for lactic acid bacteria with high inhibitory power was developed. The methodology employed was the agar-diffusion assay, which was standardized for the indicator strain, medium composition, and incubation conditions. The assay was performed in nutrient agar at 30 degrees C with 10(8) spores of Bacillus subtilis per plate as indicator strain. The inhibition produced by supernatants of lactic acid bacteria cultures harvested at stationary phase was determined. The inhibitory powers of different strains were compared with a standard curve obtained with racemic lactic acid. Results obtained with lactic acid and supernatants of bacterial cultures demonstrate that the diameter of the inhibition zone (d) was related to the pH by the exponential relation d = a exp (- b pH). Results obtained with strains that produced inhibitory substances other than lactic acid could not be fitted into the standard linear curves obtained in the plot of ln d versus pH of lactic acid.
引用
收藏
页码：739 / 745
页数：7
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