Enzymatic synthesis of thioesters in non-conventional solvents

被引：0

作者：

Caussette, M ^{[1
]}

Marty, A ^{[1
]}

Combes, D ^{[1
]}

机构：

[1] INST NATL SCI APPL,CTR BIOINGN GILBERT DURAND,UMR 5504,LA INRA,F-31077 TOULOUSE,FRANCE

来源：

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 1997年 / 68卷 / 03期

关键词：

thioesterification; thioester; lipase; n-hexane; supercritical carbon dioxide;

D O I：

10.1002/(SICI)1097-4660(199703)68:3<257::AID-JCTB619>3.0.CO;2-M

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The feasibility of enzymatic thioesterification between oleic acid and butanethiol in n-hexane, with the immobilised lipase (Lipozyme) from Mucor miehei, has been demonstrated. The immobilised enzyme quantity (100 mg), temperature (40 degrees C), pH range (6-9) and water content (10%) were studied and their optimum values were determined. A preliminary kinetic study indicated a low butanethiol affinity for the enzyme (K-m = 1 . 85 mol dm(-3)). Even when butanethiol was used without solvent, no substrate inhibition was observed. The possibility of carrying out this reaction in a natural solvent, supercritical carbon dioxide (SCCO2), was successfully verified. After 8 h reaction, a conversion yield of oleic acid of about 33% was obtained.

引用

页码：257 / 262

页数：6

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