Storage protein markers in powdery mildew resistant wheat Aegilops kotschyi introgression line

In order to identify the alien chromosome introgressed into the wheat's genome of the aneuploid lines of the cross: Triticum aestivum cv Kiten (AABBDD) x Aegilops kotschyi (UUSS) a protein polymorphism (gliadins and glutenins) as markers for 1(-st) and 6(-th) homoeologous group is used. An SDS-PAGE and a native 1-D-slab PAGE in the case of glutenins and gliagins respectively are performed. Some additional bands specific for Ae.kotschyi as well as a comparison to Ae. umbellulata (UU), Ae. longissima ((SS1)-S-1), Ae. squrrosa (DD) and Chinese Spring nullitetrasomic lines indicate the introgression of U genome in the investigated disomic addition and substitution lines. The results of this study show that the polymorphism of the storage proteins can be used as a reliable marker system for quick and cheaper screening of the presence of alien chromosomes.