Characterization of glycoengineered anti-HER2 monoclonal antibodies produced by using a silkworm-baculovirus expression system

被引：8
|
作者：
Egashira, Yuriko [1 ,2 ]
Nagatoishi, Satoru [1 ,3 ]
Kiyoshi, Masato [4 ]
Ishii-Watabe, Akiko [4 ]
Tsumoto, Kouhei [1 ,3 ]
机构：
[1] Univ Tokyo, Sch Engn, Dept Bioengn, Bunkyo Ku, Tokyo 1138656, Japan
[2] Sysmex Corp, Prot Dev Ctr, Sayama, Saitama 3501332, Japan
[3] Univ Tokyo, Inst Med Sci, Minato Ku, Tokyo 1088639, Japan
[4] Natl Inst Hlth Sci, Div Biol Chem & Biol, Kawasaki, Kanagawa 2109501, Japan
来源：
JOURNAL OF BIOCHEMISTRY | 2018年 / 163卷 / 06期
基金：
日本学术振兴会;
关键词：
baculovirus; monoclonal antibody; N-glycosylation; silkworm; trastuzumab; DEPENDENT CELLULAR CYTOTOXICITY; BETA-N-ACETYLGLUCOSAMINIDASES; LINKED GLYCAN STRUCTURES; BOMBYX-MORI; GLYCOSYLATION; PROTEIN; CELLS; LARVAE; FC; ESTABLISHMENT;
D O I：
10.1093/jb/mvy021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Silkworm-baculovirus expression systems are efficient means for the production of recombinant proteins that provide high expression levels and post-translational modifications. Here, we characterized the stability, glycosylation pattern and antibody-dependent cell-mediated cytotoxicity activity of anti-HER2 monoclonal antibodies containing native or glycoengineered mammalian-like N-glycans that were produced by using a silkworm-baculovirus expression system. Compared with a monoclonal antibody produced by using a Chinese hamster ovary cell expression system, the glycoengineered monoclonal antibody had comparable thermal stability and a higher antibody-dependent cell-mediated cytotoxicity activity. These results suggest that silkworm-baculovirus expression systems are next-generation expression systems potentially useful for the cost-effective production of therapeutic antibodies.
引用
收藏
页码：481 / 488
页数：8
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