Biological Activity of Recombinant Bovine Interferon τ Produced by a Silkworm-Baculovirus Gene Expression System

被引：2
|
作者：
Takahashi, Hitomi [1 ]
Tsunazaki, Makoto [1 ,2 ]
Hamano, Takashi [1 ,3 ]
Takahashi, Masashi [4 ]
Okuda, Kiyoshi [5 ]
Inumaru, Shigeki [6 ]
Okano, Akira [1 ]
Geshi, Masaya [1 ]
Hirako, Makoto [1 ]
机构：
[1] NARO Inst Livestock & Grassland Sci, Anim Breeding & Reprod Res Div, Tsukuba, Ibaraki 3050901, Japan
[2] Ehime Res Inst Agr, Iyo, Ehime 7971211, Japan
[3] Livestock Technol Promot Ctr, Hino, Shiga 5291651, Japan
[4] NARO Kyushu Okinawa Agr Res Ctr, Livestock & Grassland Res Div, Kumamoto 8611192, Japan
[5] Okayama Univ, Grad Sch Nat Sci & Technol, Lab Reprod Endocrinol, Okayama, Okayama 7008530, Japan
[6] Natl Inst Anim Hlth, Tsukuba, Ibaraki 3050856, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2014年 / 76卷 / 03期
关键词：
bioactivity; interferon tau; maternal-fetal recognition; recombinant; silkworm baculovirus; OVINE TROPHOBLAST PROTEIN-1; NECROSIS-FACTOR-ALPHA; ENDOMETRIAL CELLS; PROSTAGLANDIN-F2-ALPHA SYNTHESIS; STROMAL CELLS; INSECT CELLS; IN-VITRO; PURIFICATION; PREGNANCY; INVITRO;
D O I：
10.1292/jvms.12-0403
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Bovine interferon (bIFN) tau plays a crucial role in maternal-fetal recognition and was expressed using a Bombyx mari (Bm) nuclear polyhedrosis virus (silkworm baculovirus) gene expression system. The biological effects of Bm-recombinant bIFN tau (rbIFN tau) on prostaglandin (PG) F-2 alpha synthesis were investigated in cultured bovine endometrial epithelial cells with oxytocin (OT, 100 nM) and on the in vitro development of bovine embryos. Bm-rbIFN tau and OT were shown to suppress PGF(2 alpha) production in a dose-dependent manner. When in vitro produced morula stage embryos were cultured for 72 hr in modified CR1aa medium supplemented with or without rbIFN tau, Bm-rbIFN tau (10 ng/ml) significantly promoted development to the expanded blastocyst stage. In conclusion, Bm-rbIFN tau was suggested to have the same bioactivity as native IFN tau.
引用
收藏
页码：447 / 451
页数：5
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