Advanced Biological Imaging for Intracellular Micromanipulation: Methods and Applications

被引：6
|
作者：
Gao, Wendi [1 ,2 ,3 ]
Zhao, Libo [1 ,2 ]
Jiang, Zhuangde [1 ,2 ]
Sun, Dong [3 ]
机构：
[1] Xi An Jiao Tong Univ, State Key Lab Mfg Syst Engn, Sch Mech Engn, Xian 710049, Peoples R China
[2] Xi An Jiao Tong Univ, Int Joint Lab Micro Nano Mfg & Measurement Techno, Sch Mech Engn, Xian 710049, Peoples R China
[3] City Univ Hong Kong, Dept Biomed Engn, Hong Kong 999077, Peoples R China
来源：
APPLIED SCIENCES-BASEL | 2020年 / 10卷 / 20期
基金：
中国国家自然科学基金;
关键词：
biological imaging; computational imaging; intracellular micromanipulation; LIGHT-SHEET MICROSCOPY; STRUCTURED-ILLUMINATION MICROSCOPY; ATOMIC-FORCE MICROSCOPY; FLUORESCENCE MICROSCOPY; PHOTOACOUSTIC MICROSCOPY; RESOLUTION ENHANCEMENT; STIMULATED-EMISSION; SUBCELLULAR-LOCALIZATION; CONFOCAL MICROSCOPY; 2-PHOTON MICROSCOPY;
D O I：
10.3390/app10207308
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Intracellular micromanipulation assisted by robotic systems has valuable applications in biomedical research, such as genetic diagnosis and genome-editing tasks. However, current studies suffer from a low success rate and a large operation damage because of insufficient information on the operation information of targeted specimens. The complexity of the intracellular environment causes difficulties in visualizing manipulation tools and specimens. This review summarizes and analyzes the current development of advanced biological imaging sampling and computational processing methods in intracellular micromanipulation applications. It also discusses the related limitations and future extension, providing an important reference about this field.
引用
收藏
页码：1 / 34
页数：34
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