Development and Validation of a Procedure for Acyzole Determination in Whole Human Blood

被引：1
|
作者：
Kursakov, S. V. [1 ]
Belov, V. Yu. [1 ]
Sevast'yanov, V. I. [1 ,2 ]
机构：
[1] Inst Biomed Res & Technol, Moscow 123557, Russia
[2] Russian Minist Hlth, Shumakov Fed Res Ctr Transplantol & Artificial Or, Moscow 123182, Russia
来源：
PHARMACEUTICAL CHEMISTRY JOURNAL | 2014年 / 48卷 / 04期
关键词：
acyzole; determination procedure; HPLC;
D O I：
10.1007/s11094-014-1098-3
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
7An HPLC procedure using a HILIC column and UV detection was developed for determination of the carbon monoxide antidote acyzole in human blood after precipitation of proteins with MeCN. The chromatography was performed in isocratic mode over a Nucleodur HILIC column with MeCN:H2O (95:5) mobile phase and detection at 225 nm. The lower limit of quantitation was 116.2 ng/mL; the upper limit, 11,111 ng/mL. Validation of the procedure confirmed that its selectivity, carry-over, linearity, limit of quantitation, precision and accuracy, recovery, and stability satisfied the established guidelines.
引用
收藏
页码：298 / 301
页数：4
相关论文
共 50 条
  • [31] Development and validation of a simple and rapid thin-layer chromatography–UV densitometry method for the determination of triazophos in human whole blood for forensic toxicological applications
    Praveen U. Sanganalmath
    Purigali M. Nagaraju
    Kuruba Sreeramulu
    JPC – Journal of Planar Chromatography – Modern TLC, 2020, 33 : 255 - 262
  • [32] Development and validation of a high throughput whole blood thrombolysis plate assay
    T. Bonnard
    L. S. Law
    Z. Tennant
    C. E. Hagemeyer
    Scientific Reports, 7
  • [33] Development and validation of a high throughput whole blood thrombolysis plate assay
    Bonnard, T.
    Law, L. S.
    Tennant, Z.
    Hagemeyer, C. E.
    SCIENTIFIC REPORTS, 2017, 7
  • [34] Development of a laboratory model of thrombocytopenia in human whole blood
    Bercovitz, R. S.
    Brenner, M. K.
    Newman, D. K.
    JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2014, 12 : 77 - 77
  • [35] A GC–MS method for the determination of furanylfentanyl and ocfentanil in whole blood with full validation
    Nektaria Misailidi
    Sotiris Athanaselis
    Panagiota Nikolaou
    Maria Katselou
    Yannis Dotsikas
    Chara Spiliopoulou
    Ioannis Papoutsis
    Forensic Toxicology, 2019, 37 : 238 - 244
  • [36] A simple HPLC method for the determination of cyclosporin A in human whole blood
    Yang, SG
    Park, SR
    Kim, DD
    Chung, SJ
    Shim, CK
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2006, 29 (03) : 391 - 401
  • [37] Determination of strontium in human whole blood by ICP-AES
    Pietre, M.
    Desmet, B.
    Dams, R.
    Science of the Total Environment, 1994, 141 (1-3)
  • [38] HPLC determination and pharmacokinetic study of indapamide in human whole blood
    Gao, XL
    Chen, J
    Mei, N
    Tao, WX
    Jiang, WM
    Jiang, XG
    CHROMATOGRAPHIA, 2005, 61 (11-12) : 581 - 585
  • [39] HPLC Determination and Pharmacokinetic Study of Indapamide in Human Whole Blood
    Xiaoling Gao
    Jun Chen
    Ni Mei
    Weixing Tao
    Wenming Jiang
    Xinguo Jiang
    Chromatographia, 2005, 61 : 581 - 585
  • [40] THE DEVELOPMENT AND VALIDATION OF A LC-MS/MS METHOD FOR THE DETERMINATION OF LUMEFANTRINE AND DESBUTYL LUMEFANTRINE IN WHOLE BLOOD ON SAMPLING PAPER
    Smit, I.
    Castel, S.
    Norman, J.
    Smit, P.
    Wiesner, L.
    BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY, 2014, 115 : 278 - 279
12345