The analysis of thyroxine in human serum by an 'exact matching' isotope dilution method with liquid chromatography/tandem mass spectrometry

被引：37
|
作者：
Hopley, CJ
Stokes, P
Webb, KS
Baynham, M
机构：
[1] Lab Govt Chemist, Teddington TW11 0LY, Middx, England
[2] Appl Biosyst Inc, Warrington WA3 7QH, Cheshire, England
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2004年 / 18卷 / 10期
关键词：
D O I：
10.1002/rcm.1441
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the analysis of thyroxine in human serum, utilising 'exact matching' isotope dilution mass spectrometry (IDMS) in combination with liquid chromatography/tandem mass spectrometry (LC/MS/MS), has been developed with a limit of quantification of 0.5 ng g(-1) of thyroxine in human serum. The extraction and clean-up of thyroxine from serum involves an efficient protein-precipitation stage followed by a solid-phase extraction procedure to produce an extract essentially free from interfering compounds. The method is reproducible, with expanded uncertainties of less than 2%, and is relatively fast to perform. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码：1033 / 1038
页数：6
相关论文
共 50 条
  • [41] Quantitative analysis of cholesterol in infant formula by isotope dilution liquid chromatography-tandem mass spectrometry
    Ahn, Eun Jeong
    Lee, Hwa Shim
    Kim, Byung Joo
    Lee, Gae Ho
    ANALYTICAL SCIENCE AND TECHNOLOGY, 2015, 28 (06): : 460 - 466
  • [42] Determination of total cholesterol in serum by liquid chromatography isotope dilution mass spectrometry
    Kock, R
    Delvoux, B
    Greilling, H
    CLINICAL CHEMISTRY, 1997, 43 (10) : 1896 - 1903
  • [43] Determination of aflatoxins in animal feeds by liquid chromatography/tandem mass spectrometry with isotope dilution
    Li, Wei
    Herrman, Timothy J.
    Dai, Susie Y.
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2011, 25 (09) : 1222 - 1230
  • [44] Quantification of intracellular homocysteine by stable isotope dilution liquid chromatography/tandem mass spectrometry
    Huang, Yuehua
    Lu, Zhi-Yong
    Brown, Karen S.
    Whitehead, Alexander S.
    Blair, Ian A.
    BIOMEDICAL CHROMATOGRAPHY, 2007, 21 (01) : 107 - 112
  • [45] Measurement of serum progesterone by isotope dilution liquid chromatography tandem mass spectrometry: a candidate reference method and its application to evaluating immunoassays
    Yufei Wang
    Tianjiao Zhang
    Haijian Zhao
    Weiyan Zhou
    Jie Zeng
    Jiangtao Zhang
    Chuanbao Zhang
    Wenxiang Chen
    Analytical and Bioanalytical Chemistry, 2019, 411 : 2363 - 2371
  • [46] Measurement of serum progesterone by isotope dilution liquid chromatography tandem mass spectrometry: a candidate reference method and its application to evaluating immunoassays
    Wang, Yufei
    Zhang, Tianjiao
    Zhao, Haijian
    Zhou, Weiyan
    Zeng, Jie
    Zhang, Jiangtao
    Zhang, Chuanbao
    Chen, Wenxiang
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2019, 411 (11) : 2363 - 2371
  • [47] Determination of serum creatinine by liquid chromatography isotope dilution tandem mass spectrometry (LC-IDMS/MS): A candidate reference method
    Rasoul, Alikhani-Koupaei
    Mehrdad, Yazdanpanah
    Eleftherios, Diamandis
    Barry, Hoffman
    Khosrow, Adeli
    Jeffrey, Tong
    CLINICAL BIOCHEMISTRY, 2008, 41 (14-15) : 1273 - 1273
  • [48] Comparison of gas chromatography and liquid chromatography mass spectrometric measurements for high accuracy analysis of cholesterol in human serum by isotope dilution mass spectrometry
    Briche, CSJW
    Carter, D
    Webb, KS
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (09) : 848 - 853
  • [49] Development and validation of a rapid liquid chromatography isotope dilution tandem mass spectrometry (LC-IDMS/MS) method for serum creatinine
    Hetu, Pierre-Olivier
    Gingras, Marie-Eve
    Vinet, Bernard
    CLINICAL BIOCHEMISTRY, 2010, 43 (13-14) : 1158 - 1162
  • [50] Quantification of glycocholic acid in human serum by stable isotope dilution ultra performance liquid chromatography electrospray ionization tandem mass spectrometry
    Guo, Cheng
    Xie, Cong
    Ding, Peili
    Qin, Guangming
    Mo, Weimin
    Cao, Xiaoji
    Zheng, Shu
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2018, 1072 : 315 - 319
12345