Dynamic effects of leflunomide on IL-1, IL-6, and TNF-α activity produced from peritoneal macrophages in adjuvant arthritis rats

被引：0

作者：

Li, WD ^{[1
]}

Ran, GX ^{[1
]}

Teng, HL ^{[1
]}

Lin, ZB ^{[1
]}

机构：

[1] Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Beijing 100083, Peoples R China

来源：

ACTA PHARMACOLOGICA SINICA | 2002年 / 23卷 / 08期

关键词：

leflunomide; interleukin-1; interleukin-6; tumor necrosis factor; macrophages; adjuvant arthritis;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

AIM: To investigate the effects of leflunomide (LEF) on modulating interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor- alpha (TNF-alpha) production induced by lipopolysaccharide (LPS) in peritoneal macrophages (PMphi) in adjuvant arthritis rats and elucidate the possible mechanisms of antiinflammatory and antirheumatoid effects of LEF. METHODS: Freund's complete adjuvant was injected in the hind footpad of rats to induce adjuvant arthritis (AA) rat model. The PMphi samples were taken at different time after medication. IL-1, IL-6, and TNF-alpha activities released from PMphi were measured by ELISA method or bioassay method. RESULTS: Production of IL-1, IL-6, and TNF-alpha was increased in the culture supernatant of PMphi in AA model rat. LEF could inhibit LPS-induced release of IL-1 and TNF-alpha from PMphi of the AA rats and the inhibitory effects were extremely rapid. LEF (10, 25 mg/kg) administrated for 21d could inhibit IL-6 release from PMphi in AA rats. CONCLUSION: The antiinflammatory mechanisms of LEF in AA rats might be related to inhibitory level of IL-1, IL-6, and TNF-alpha from PMphi in vivo.

引用

页码：752 / 756

页数：5

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