Non-linear optical microscopy of kidney tumours

被引：28

作者：

Galli, Roberta ^{[1
]}

Sablinskas, Valdas ^{[2
]}

Dasevicius, Darius ^{[3
]}

Laurinavicius, Arvydas ^{[4
,5
]}

Jankevicius, Feliksas ^{[6
]}

Koch, Edmund ^{[1
]}

Steiner, Gerald ^{[1
]}

机构：

[1] Tech Univ Dresden, Fac Med Clin Sensing & Monitoring, D-01307 Dresden, Germany

[2] Vilnius Univ, Fac Phys, LT-10222 Vilnius, Lithuania

[3] Natl Ctr Pathol, LT-08406 Vilnius, Lithuania

[4] Vilnius Univ, Fac Med, LT-08661 Vilnius, Lithuania

[5] Natl Ctr Pathol, LT-08661 Vilnius, Lithuania

[6] Vilnius Univ, Vilnius Univ Hosp, Santariskiu Klin, LT-08661 Vilnius, Lithuania

来源：

JOURNAL OF BIOPHOTONICS | 2014年 / 7卷 / 1-2期

关键词：

non-linear microscopy; kidney; cancer; CARS; TPEF; SHG; label-free; SCATTERING; EPIDEMIOLOGY; BIOLOGY;

D O I：

10.1002/jbio.201200216

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The unregulated cancer cell growth leads to strong alterations in morphology and composition of the tissue. The combination of coherent anti-Stokes Raman scattering, two-photon excited fluorescence and second harmonic generation enables a high resolution imaging with strong information on tissue composition and can then provide useful information for tumour diagnosis. Here we present the potential of multimodal non-linear microscopy for imaging of renal tumours. Using cryosections of human oncocytoma and carcinoma, the method gave a detailed insight in cancer morphology and composition, enabling to discern between normal kidney tissue, tumour and necrosis. Several features significant for the diagnosis were clearly visualised without use of any staining. Translation of this method in clinical pathology will greatly improve speed and quality of the analyses. ((c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

引用

页码：23 / 27

页数：5

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