A simple twist for signal enhancement in non-linear optical microscopy

被引：4
|
作者：
De, A. K. [1 ]
Goswami, D. [1 ]
机构：
[1] Indian Inst Technol, Dept Chem, Kanpur 208016, Uttar Pradesh, India
来源：
JOURNAL OF MICROSCOPY | 2009年 / 235卷 / 02期
基金：
英国惠康基金;
关键词：
Cellular imaging; continuous wave and pulsed excitation; fluorescence enhancement; laser-scanning microscopy; two-photon absorption; 2-PHOTON; ABSORPTION; EXCITATION;
D O I：
10.1111/j.1365-2818.2009.03176.x
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
We describe a very simple but elegant approach to two-photon fluorescence signal enhancement by intensity modulation with immediate application in two-photon laser-scanning fluorescence microscopy. This method of enhancement shows potential application in any microscopic technique that result from non-linear photon absorption and plays a pivotal role in live cell imaging.
引用
收藏
页码：119 / 123
页数：5
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