Single molecule studies of DNA binding proteins using optical tweezers

被引：12
|
作者：
Kimura, Yuji
Bianco, Piero R. [1 ]
机构：
[1] SUNY Buffalo, Ctr Single Mol Biophys, Buffalo, NY 14214 USA
[2] SUNY Buffalo, Dept Microbiol & Immunol, Buffalo, NY 14214 USA
[3] SUNY Buffalo, Dept Biochem, Buffalo, NY 14214 USA
来源：
ANALYST | 2006年 / 131卷 / 08期
关键词：
D O I：
10.1039/b600157m
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Optical tweezers have become a versatile tool in the biological sciences. Combined with various types of optical microscopy, they are being successfully used to discover the fundamental mechanism of biological processes. Recently, the study of proteins acting on DNA was aggressively undertaken at the single-molecule level. Here, we review the most recent studies which have revealed the dynamic behavior of individual protein molecules at work on DNA, providing detailed mechanistic insight that could not be revealed, at least not easily, using bulk-phase or ensemble approaches.
引用
收藏
页码：868 / 874
页数：7
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