Porous silicon used as support for protein microarray

被引：6
|
作者：
Simion, M. [1 ]
Ruta, L. [2 ]
Mihailescu, C. [3 ]
Kleps, I. [1 ]
Bragaru, A. [1 ]
Miu, M. [1 ]
Ignat, T. [1 ]
Baciu, Ion [2 ]
机构：
[1] Natl Inst Res & Dev Microtechnol IMT, Bucharest 077190, Romania
[2] Univ Bucharest, Bucharest 00133, Romania
[3] DDS Diagnost Bucharest, Bucharest, Romania
来源：
SUPERLATTICES AND MICROSTRUCTURES | 2009年 / 46卷 / 1-2期
关键词：
Porous silicon; Protein immobilization; Technology microrarray; SERUM-ALBUMIN;
D O I：
10.1016/j.spmi.2008.11.024
中图分类号：
O469 [凝聚态物理学];
学科分类号：
070205 ;
摘要：
Porous silicon (PS) substrate was performed and investigated as a substrate for protein attachment. Two porous silicon substrates were fabricated by anodic dissolution of the silicon wafers p type, (111) and (100) crystalline orientation in hydrofluoric acid (HF). Different pore sizes and depths were accomplished by varying the current and processing time and also electrolyte composition. Serum Bovine Albumin (BSA) labels with CY3 was printed on commercial glass for microarray and PS samples in controlled conditions of humidity and temperature. After a standard incubation time (12 h) the morphology of microarray substrates were examined by scanning electron microscopy (SEM) Analysis. The uniformity of spot and linearity of the signal intensity was investigated by florescent laser scanning. (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：69 / 76
页数：8
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