Next generation of protein microarray support materials:: Evaluation for protein and antibody microarray applications

被引：156
|
作者：
Angenendt, P
Glökler, J
Sobek, J
Lehrach, H
Cahill, DJ
机构：
[1] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
[2] Royal Coll Surgeons Ireland, Natl Ctr Human Proteon, Dublin 2, Ireland
[3] Funct Genom Ctr Zurich, CH-8057 Zurich, Switzerland
[4] Microsynth GmbH, CH-9436 Balgach, Switzerland
[5] PROTAGEN, D-44227 Dortmund, Germany
来源：
JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1009卷 / 1-2期
关键词：
chip technology; proteins; antibodies;
D O I：
10.1016/S0021-9673(03)00769-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The performance of protein and antibody microarrays is dependent on various factors, one of which is the use of an appropriate microarray surface for the immobilisation of either protein or antibody samples. We have investigated the properties of seven new surfaces in the context of both protein and antibody microarray technology. We have demonstrated the functionality of all new slide coatings and investigated the mean signal to spotted concentration ratio, determined detection limits and calculated coefficients of variation. Moreover, new concepts for slide coatings such as dendrimer and poly(ethylene glycol)-epoxy slides were evaluated and improved qualities of novel slide surfaces were observed. Optimal slide coatings for antibody and protein chips were proposed and the requirements for both technologies were discussed. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码：97 / 104
页数：8
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